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首页> 外文期刊>The Journal of automatic chemistry >Development of a Simple and Powerful Analytical Method for Formaldehyde Detection and Quantitation in Blood Samples
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Development of a Simple and Powerful Analytical Method for Formaldehyde Detection and Quantitation in Blood Samples

机译：血液样本中甲醛检测和定量的一种简单而强大的分析方法的发展

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Human beings are easily exposed to formaldehyde (FA) in a living environment. Entry of FA into the human body can have adverse effects on human health, depending on the FA concentration. Thus, a quantitative analysis of FA in blood is necessary in order to estimate its effect on the human body. In this study, a simple and rapid analytical method for the quantitation of FA in blood was developed. The total analysis time, including the pretreatment procedure, was less than 20?min. To ensure a stable analysis, blood samples were stabilized using tripotassium ethylenediaminetetraacetic acid solution, and FA was selectively derivatized using 2,4-dinitrophenylhydrazine as pretreatment procedures. The pretreated samples were analyzed using a high-performance liquid chromatography-UV system, which is the most common choice for analyzing small-molecule aldehydes like formaldehyde. Verification of the pretreatment methods (stabilization and derivatization) using FA standards confirmed that the pretreatment methods are highly reliable in the calibration range 0.012–5.761?ng? μ L~(–1) (slope?=?684,898, R ~(2)?=?0.9998, and limit of detection?=?0.251?pg· μ L~(–1)). Analysis of FA in the blood samples of a Yucatan minipig using the new method revealed an average FA concentration of 1.98?±?0.34?ng? μ L~(–1) ( n ?=?3). Blood samples spiked with FA standards were analyzed, and the FA concentrations were found to be similar to the theoretical concentrations (2.16?±?0.81% difference). The method reported herein can quantitatively analyze FA in blood at a sub-nanogram level within a short period of time and is validated for application in blood analysis.
机译：人类在生活环境中容易暴露于甲醛（FA）。根据FA浓​​度，FA进入人体的进入人体对人体的影响。因此，必须在血液中的定量分析是必要的，以估计其对人体的影响。在这项研究中，开发了一种简单而快速的分析方法，用于定量血液中的FA。总分析时间（包括预处理程序）小于20？min。为了确保稳定的分析，使用乙二胺四乙酸溶液稳定血液样品，并选择性地使用2,4-二硝基苯基肼作为预处理程序选择性衍生。使用高性能液相色谱 - UV系统分析预处理样品，这是分析甲醛等小分子醛的最常见的选择。使用FA标准验证预处理方法（稳定和衍生化）证实，预处理方法在校准范围内高度可靠，0.012-5.761？ μl〜（-1）（斜率？=Δ= 684,898，r〜（2）？= 0.9998，以及检测限Δ= 0.251？pg·μl〜（1））。使用新方法分析尤卡坦MINIPIG的血液样本中的血液样本，揭示了1.98？±0.34？ng？ μl〜（-1）（n？=？3）。分析了用FA标准掺入的血液样品，发现FA浓度类似于理论浓度（2.16≤≤0.81％）。本文报道的方法可以在短时间内定量分析血液中的血液中的FA，并验证用于血液分析中的应用。

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