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首页> 外文期刊>Journal of Molecular Genetics >A Consensus Sequence among Natural Transmembrane Linkers for Single-Polypeptide Chain (SC) Connection of Two G-Protein-Coupled Receptors in Tandem
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A Consensus Sequence among Natural Transmembrane Linkers for Single-Polypeptide Chain (SC) Connection of Two G-Protein-Coupled Receptors in Tandem

机译：用于单多肽链（SC）连接的自然跨膜接头之间的共有序列，其两种G蛋白偶联受体的连接

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摘要

A transmembrane (TM) single-polypeptide chain (sc) linker that can connect any two class A Gprotein-coupled receptors (GPCRs) in tandem as a genefusion strategy has been demonstrated. Here, we report that a natural TM linker for sc exists that can connect two GPCRs in tandem. In silico analysis indicated that the central intervening portion (amino acid residues 311~416) of Exaiptasia diaphana (LOC110241027) GPCR fusion protein contains a single probable TM domain and that a consensus sequence, T(I/A/P) (A/S) (L/N) (I/W/L) (I/A/V) GL (L/G) (A/T) (S/L/G) (I/L) is conserved between this TM and previously characterized TM-linkers deriving from the type II TM proteins with a cytoplasmic N-ter segment, single TM and extracellular C-ter tail, Caenorhabditis elegans odr4 and human CD23. In addition, to the invertebrate TM-linker, it was found that in the central intervening region of tropical bird Opisthocomus hoazin protein LOC104327099 (XP_009930279.1), TM-linker contains the consensus sequence. Here, we directed for the first time, our attention to the natural TM sc linker as a rare evolutionary event among all mammalian and avian proteins.
机译：已经证明了可以将任何两类GProtein偶联受体（GPCR）连接为基因反映策略的跨膜（TM）单多肽链（SC）接头。在这里，我们报告称SC的自然TM链接器存在，可以在串联中连接两个GPCR。在硅分析中，表明exapipaIpiaia癫痫（Loc110241027）GPCR融合蛋白的中央介入部分（氨基酸残基311〜416）含有单个可能的TM结构域，并且共有序列T（I / A / P）（A / S. ）（L / N）（I / W / L）（I / A / V）GL（L / G）（A / L / v）（S / L / g）（I / L）在该TM和以前保守表征从II型TM蛋白质的TM接头，具有细胞质N-TER段，单TM和细胞外C-TER尾，CaenorhabditisEgiss ODR4和人CD23。此外，对于无脊椎动物TM-Linker，发现在热带鸟类Opisthocomus Hoazin蛋白LOC104327099（XP_009930279.1）中，TM-Linker含有共有序列。在这里，我们首次引导，我们注意自然TM SC链接器作为所有哺乳动物和禽蛋白之间的罕见进化事件。

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