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首页> 外文期刊>Applied Microbiology >Chemical Targeting and Manipulation of Type III Secretion in the Phytopathogen Xanthomonas campestris for Control of Disease
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Chemical Targeting and Manipulation of Type III Secretion in the Phytopathogen Xanthomonas campestris for Control of Disease

机译：III型分泌植物植物病原Xanthomonas Campestris中的化学靶向和操纵治疗疾病

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Xanthomonas campestris pv. campestris is the causative agent of black rot disease in crucifer plants. This Gram-negative bacterium utilizes the type III secretion system (T3SS), encoded by the hrp gene cluster, to aid in its resistance to host defenses and the ability to cause disease. The T3SS injects a set of proteins known as effectors into host cells that come into contact with the bacterium. The T3SS is essential for the virulence and hypersensitive response (HR) of X. campestris pv. campestris, making it a potential target for disease control strategies. Using a unique and straightforward high-throughput screening method, we examined a large collection of diverse small molecules for their potential to modulate the T3SS without affecting the growth of X. campestris pv. campestris. Screening of 13,129 different compounds identified 10 small molecules that had a significant inhibitory influence on T3SS. Moreover, reverse transcription-quantitative PCR (qRT-PCR) assays demonstrated that all 10 compounds repress the expression of the hrp genes. Interestingly, the effect of these small molecules on hrp genes may be through the HpaS and ColS sensor kinase proteins that are key to the regulation of the T3SS in planta . Five of the compounds were also capable of inhibiting X. campestris pv. campestris virulence in a Chinese radish leaf-clipping assay. Furthermore, seven of the small molecules significantly weakened the HR in nonhost pepper plants challenged with X. campestris pv. campestris. Taken together, these small molecules may provide potential tool compounds for the further development of antivirulence agents that could be used in disease control of the plant pathogen X. campestris pv. campestris.IMPORTANCE The bacterium Xanthomonas campestris pv. campestris is known to cause black rot disease in many socioeconomically important vegetable crops worldwide. The management and control of black rot disease have been tackled with chemical and host resistance methods with variable success. This has motivated the development of alternative methods for preventing this disease. Here, we identify a set of novel small molecules capable of inhibiting X. campestris pv. campestris virulence, which may represent leading compounds for the further development of antivirulence agents that could be used in the control of black rot disease.
机译：Xanthomonas Campestris PV。 Campestris是十字花植物植物中黑腐病的致病因子。该革兰阴性细菌利用由HRP基因簇编码的III型分泌系统（T3S），以帮助其对宿主防御的抵抗力和引起疾病的能力。 T3SS注入一组称为效应器的蛋白质，进入与细菌接触的宿主细胞中。 T3S对于X.Campestris PV的毒力和过敏反应（HR）至关重要。 Campestris，使其成为疾病控制策略的潜在目标。使用独特和直接的高通量筛选方法，我们检查了大量不同的小分子，以便它们调节T3SS而不影响X.Campestris PV的生长。 Campestris。筛选13,129种不同的化合物鉴定了10个小分子，对T3Ss具有显着的抑制作用。此外，逆转录定量PCR（QRT-PCR）测定证明所有10种化合物抑制了HRP基因的表达。有趣的是，这些小分子对HRP基因对HTP基因的影响可以通过HPA和COLS传感器激酶蛋白，它们是植物中T3SS调节的关键。其中五种化合物也能够抑制X.Campestris PV。在中国萝卜叶剪切测定中的Campestris毒业。此外，七种小分子明显削弱了与X.Campestris PV挑战的非体式辣椒植物中的人力资源。 Campestris。总之，这些小分子可以提供潜在的工具化合物，用于进一步发展抗血管原剂，其可用于植物病原体X.Campestris PV的疾病控制。 Campestris.portance细菌Xanthomonas Campestris PV。众所周知，Campestris在全球许多社会经济上重要蔬菜作物中引起黑色腐败疾病。用可变成功的化学和宿主抗性方法解决了黑腐病的管理和控制。这有动力发展防止这种疾病的替代方法。在这里，我们鉴定了一组能够抑制X.Campestris PV的新型小分子。 Campestris毒力，其可以代表用于进一步发展抗血管疾病的前导化合物，其可用于控制黑腐病的控制。

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