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首页> 外文期刊>Applied Microbiology >Monitoring Microbial Populations and Antibiotic Resistance Gene Enrichment Associated with Arctic Waste Stabilization Ponds
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Monitoring Microbial Populations and Antibiotic Resistance Gene Enrichment Associated with Arctic Waste Stabilization Ponds

机译：监测与北极废物稳定池相关的微生物群和抗生素抗性基因富集

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Wastewater management in the Canadian Arctic is challenging due to climate extremes, small population sizes, and lack of conventional infrastructure for wastewater treatment. Although many northern communities use waste stabilization ponds (WSPs) as their primary form of wastewater treatment, few studies have explored WSP microbial communities and assessed effluent impacts on receiving waters from a microbiological perspective. Here, we used 16S rRNA gene and metagenome sequencing to characterize WSP and receiving water microbial communities for two time points bracketing the spring WSP thaw in Baker Lake (Nunavut) and compared these results to other Nunavut WSPs in Cambridge Bay and Kugluktuk. Most amplicon sequence variants (ASVs) recovered from these WSP samples belonged to the phylum Proteobacteria , with considerable variation between the three locations and only six ASVs shared among the WSPs at >0.2% relative abundance. Wastewater indicator ASVs for the Baker Lake WSP were identified, and few indicator ASVs were detected in samples originating from other upstream or downstream sites. The metagenomic data revealed a strong enrichment of antibiotic resistance genes for WSP samples relative to downstream and reference samples, especially for genes associated with macrolide resistance. Together, our results provide a baseline characterization for WSP microbial communities, demonstrate how indicator ASVs can be used to monitor attenuation and dilution of effluent microorganisms, and reveal that WSPs can serve as hot spots for antibiotic resistance genes.IMPORTANCE Given that the microbial communities of Arctic waste stabilization ponds (WSPs) are poorly studied to date, our characterization of multiple WSP systems and time points provides important baseline data that will assist with ongoing monitoring of effluent impacts on downstream aquatic ecosystems in the Arctic. This research also identifies indicator amplicon sequence variants (ASVs) of WSPs that will be helpful for future monitoring for WSP effluent attenuation and demonstrates that WSP microbial communities are enriched in antibiotic resistance genes. Given operational and infrastructure changes anticipated for wastewater treatment systems in the Arctic, baseline data such as these are essential for further development of safe and effective wastewater treatment systems.
机译：加拿大北极的废水管理是由于气候极端，人口尺寸小，缺乏污水处理的传统基础设施而挑战。虽然许多北部群落使用废物稳定池（WSP）作为其主要形式的废水处理，但很少有研究探索了WSP微生物社区，并评估了从微生物神经角度接受水域接受水的污水影响。在这里，我们使用了16S rRNA基因和Metagenome测序，以表征WSP和接收水微生物社区的两个时间点，在Baker Lake（Nunavut）中的春天WSP解冻，并将这些结果与剑桥湾和Kugluktuk的其他Nunavut WSP进行了比较。从这些WSP样品中回收的大多数扩增子序列变体（ASV）属于植物植物，在三个位置之间具有相当大的变异，并且在WSP之间仅为60％的相对丰度之间共享的六个ASV。鉴定了Baker Lake WSP的废水指示器ASV，并且在源自上游或下游部位的样品中检测到少数指标ASV。偏见组数据揭示了相对于下游和参考样品的WSP样品的抗生素抗性基因的强烈富集，特别是对于与大环内酯抗性相关的基因。我们的结果一起为WSP微生物群落提供了基线表征，证明了指标ASV的用途如何监测衰减和稀释流出物微生物，并且揭示了WSP可以作为抗生素抗性基因的热点。传输给予微生物群体北极废物稳定池（WSP）迄今为止，我们对多个WSP系统和时间点的表征提供了重要的基线数据，可以帮助持续监测北极地下下游水生生态系统的流出影响。该研究还识别WSP的指标扩增子序列变体（ASV），有助于对WSP流出物衰减的未来监测，并证明富含WSP微生物群落富含抗生素抗性基因。鉴于北极中的废水处理系统预期的运营和基础设施变化，基线数据，这些数据对于进一步发展安全有效的废水处理系统至关重要。

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