...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Applied Microbiology >Severe Corrosion of Carbon Steel in Oil Field Produced Water Can Be Linked to Methanogenic Archaea Containing a Special Type of [NiFe] Hydrogenase
【24h】

Severe Corrosion of Carbon Steel in Oil Field Produced Water Can Be Linked to Methanogenic Archaea Containing a Special Type of [NiFe] Hydrogenase

机译：油田中碳钢的严重腐蚀产生的水可以与含有特殊类型的[niFe]氢酶的甲状腺原煤连接

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Methanogenic archaea have long been implicated in microbially influenced corrosion (MIC) of oil and gas infrastructure, yet a first understanding of the underlying molecular mechanisms has only recently emerged. We surveyed pipeline-associated microbiomes from geographically distinct oil field facilities and found methanogens to account for 0.2 to 9.3% of the 16S rRNA gene sequencing reads. Neither the type nor the abundance of the detected methanogens was correlated with the perceived severity of MIC in these pipelines. Using fluids from one pipeline, MIC was reproduced in the laboratory, both under stagnant conditions and in customized corrosion reactors simulating pipeline flow. High corrosion rates (up to 2.43?mm Fe~(0) · yr~(?1)) with macroscopic, localized corrosion features were attributed to lithotrophic, mesophilic microbial activity. Other laboratory tests with the same waters yielded negligible corrosion rates (<0.08?mm Fe~(0) · yr~(?1)). Recently, a novel [NiFe] hydrogenase from Methanococcus maripaludis strain OS7 was demonstrated to accelerate corrosion. We developed a specific quantitative PCR (qPCR) assay and detected the gene encoding the large subunit of this hydrogenase (labeled micH ) in corrosive (>0.15?mm Fe~(0) · yr~(?1)) biofilms. The micH gene, on the other hand, was absent in noncorrosive biofilms, despite an abundance of methanogens. Reconstruction of a nearly complete Methanococcus maripaludis genome from a highly corrosive mixed biofilm revealed micH and associated genes in nearly identical genetic configuration to that in strain OS7, thereby supporting our hypothesis that the encoded molecular mechanism contributed to corrosion. Lastly, the proposed MIC biomarker was detected in multiple oil fields, indicating a geographically widespread involvement of this [NiFe] hydrogenase in MIC.IMPORTANCE Microorganisms can deteriorate built environments, which is particularly problematic in the case of pipelines transporting hydrocarbons to industrial end users. MIC is notoriously difficult to detect and monitor and, as a consequence, is a particularly difficult corrosion mechanism to manage. Despite the advent of molecular tools and improved microbial monitoring strategies for oil and gas operations, specific underlying MIC mechanisms in pipelines remain largely enigmatic. Emerging mechanistic understanding of methanogenic MIC derived from pure culture work allowed us to develop a qPCR assay that distinguishes technically problematic from benign methanogens in a West African oil field. Detection of the same gene in geographically diverse samples from North America hints at the widespread applicability of this assay. The research presented here offers a step toward a mechanistic understanding of biocorrosion in oil fields and introduces a binary marker for (methanogenic) MIC that can find application in corrosion management programs in industrial settings.
机译：甲烷型古亚菊在微生物影响石油和天然气基础设施的微血管腐蚀（MIC）中，最近才出现了对潜在的分子机制的第一次理解。我们从地理上不同的油田设施进行调查的管道相关的微生物，并发现甲烷酮占16S rRNA基因测序读数的0.2％至9.3％。检测到的甲烷的类型和丰度与这些管道中的MIC的感知严重程度都不相关。使用来自一个管道的流体，MIC在实验室中再现在实验室，包括在停滞条件下和模拟管道流动的定制腐蚀反应器中。具有宏观，局部腐蚀特征的高腐蚀速率（高达2.43？mm Fe〜（0）·Yr〜（α1））归因于碎石的嗜培素微生物活性。具有相同水域的其他实验室测试产生可忽略不计的腐蚀速率（<0.08≤mmfe〜（0）·Yr〜（？1））。最近，对来自甲基甲基球菌的新型[NiFe]氢酶进行证明，以加速腐蚀。我们开发了一种特异的定量PCR（QPCR）测定，并检测了在腐蚀性（>0.15ΩmmF〜（0）·Yr〜（0）·Yr〜（0）·Yr〜（0）·Yr〜（α1））生物膜中编码该氢酶（标记的MICH）大亚基的基因。另一方面，尽管有丰富的甲烷酮，但另一方面，在非腐蚀性生物膜中不存在MICH基因。从高度腐蚀性的混合生物膜重建几乎完全的甲烷基球菌基因组，揭示了几乎相同的遗传构型的MICH和相关基因，从而支持了我们的假设，即编码的分子机制有助于腐蚀。最后，在多个油田中检测到所提出的麦克风生物标志物，表明该[NiFe]氢酶在MIC的地理上普遍涉及.Importance微生物可以劣化的内置环境，这在向工业最终用户输送烃的管道的情况下尤其有问题。麦克风难以检测和监测，并且因此是一种特别难以管理的腐蚀机制。尽管分子工具的出现以及改善的石油和天然气操作的微生物监测策略，但管道中的具体潜在麦克风机制仍然很重要。新兴机械理解来自纯文化工作的甲状腺MIC使我们能够开发一种QPCR测定，以区分从西非油田中的良性甲烷基因的技术问题。在该测定的广泛适用性中检测来自北美暗示的地理位置样本中的相同基因。本发明提出的研究提供了对油田的生物腐蚀的机械理解的一步，并为（甲状腺原）麦克风引入了可以在工业环境中腐蚀管理计划中的应用。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号