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首页> 外文期刊>PLoS One >Candida albicans exhibit two classes of cell surface binding sites for serum albumin defined by their affinity, abundance and prospective role in interkingdom signalling
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Candida albicans exhibit two classes of cell surface binding sites for serum albumin defined by their affinity, abundance and prospective role in interkingdom signalling

机译：Candida albicans表现出两类细胞表面结合位点，用于血清白蛋白，由其亲和力，丰度和临床信号传导中的前瞻性作用定义

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摘要

Serum albumin binding to the yeast form of Candida albicans is described. Two populations of binding site are identified using two complementary spectroscopic techniques: an extrinsic fluorescent probe, 3-hexa-decanoyl-7-hydrocoumarin ([HEXCO) added to the C. albicans yeast cell surface that records the electrostatic surface potential and so responds to the surface binding of serum albumin and secondly a light scattering technique that reveals how albumin modulates aggregation of the yeast population. The albumin binding sites are found to possess different binding affinities and relative abundance leading to different total binding capacities. These are characterized as a receptor population with high affinity binding (Kd ~ 17 μM) but relatively low abundance and a separate population with high abundance but much lower affinity (Kd ~ 364 μM). The low-affinity binding sites are shown to be associated with the yeast cell aggregation. These values are found be dependent on the C . albicans strain and the nature of the culture media; some examples of these effects are explored. The possible physiological consequences of the presence of these sites are speculated in terms of evading the host’s immune response, biofilm formation and possible interkingdom signaling processes.
机译：描述了血清白蛋白与酵母形式的念珠菌肽形式的结合。使用两种互补的光谱技术鉴定了两种结合位点的群体：外部荧光探针，3-六甲基 - 癸酰-7-水加工素（加入到C. albicans酵母细胞表面上，记录静电表面电位，所以响应血清白蛋白的表面结合，其次是揭示白蛋白如何调节酵母群聚集的光散射技术。发现白蛋白结合位点具有不同的结合亲和力和相对丰度，导致不同的总结合能力。这些表征为具有高亲和力结合（KD〜17μm）但相对较低的丰度和具有高丰度但亲和力（KD〜364μm）的单独群体的受体群。低亲和力结合位点显示与酵母细胞聚集有关。找到这些值取决于C. albicans菌株和培养基的性质;探索了这些效果的一些例子。就疏忽宿主的免疫应答，生物膜形成和可能的互相信导过程来推测这些位点存在的可能生理后果。

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