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首页> 外文期刊>Japanese journal of infectious diseases >Prevalence of High-Level Aminoglycoside Resistance and Genes Encoding Aminoglycoside-Modifying Enzymes in Enterococcus faecalis and Enterococcus faecium Isolated in a University Hospital in Tokyo
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Prevalence of High-Level Aminoglycoside Resistance and Genes Encoding Aminoglycoside-Modifying Enzymes in Enterococcus faecalis and Enterococcus faecium Isolated in a University Hospital in Tokyo

机译：东京大学医院肠球菌粪粪粪粪粪豆植物和肠球菌粪便患者患有氨基糖苷酱改性酶的高水平氨基糖苷类抗性及基因的患病率

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High-level aminoglycoside resistance (HLAR) limits treatment options for invasive enterococcal infections. We examined the prevalence of HLAR, carriage of genes encoding aminoglycoside-modifying enzymes, and production of β-lactamase using the disk diffusion method, polymerase chain reaction, and a nitrocefin-based test, respectively, in Enterococcus faecalis and Enterococcus faecium isolated from patients at a university hospital in Tokyo in 2010. Of the 100 E. faecalis isolates analyzed, 30 isolates had high-level resistance (HLR) to gentamicin, and 22 isolates had HLR to streptomycin. Of the 40 E. faecium isolates analyzed, 9 isolates had HLR to gentamicin, and 9 isolates had HLR to streptomycin. Of the 39 gentamicin-HLR enterococcal isolates, 24 isolates were non-HLR to streptomycin. All 39 isolates with HLR to gentamicin as well as 19 of 101 without HLR carried aac(6′)-Ie-aph(2′′)-Ia . Carriage of ant(6′)-Ia was confirmed in 25 of 31 streptomycin-HLR isolates. Production of β-lactamase was documented in none of the E. faecalis and E. faecium isolates. Whole-genome sequencing analysis revealed that all but one E. faecalis isolate that carried aac(6′)-Ie-aph(2′′)-Ia and ant(6′)-Ia belonged to sequence type (ST) 4 ( n = 8), ST16 ( n = 4), or ST179 ( n = 9). Nevertheless, most of the pairs of isolates had 10 single-nucleotide polymorphisms even among the isolates of the same ST.
机译：高水平的氨基糖苷抗性（HLAR）限制了侵入性肠球菌感染的治疗选择。我们研究了Hlar的患病率，编码氨基糖苷改性酶的基因，以及使用磁盘扩散方法，聚合酶链反应和基于硝基芬的试验的β-内酰胺酶的产生，分别在肠球菌和肠球菌粪便中患者分离出来在2010年在东京的大学医院。分析的100e.粪便分离株分析，30分离物对庆大霉素具有高水平抗性（HLR），22个分离物具有HLR至链霉素。在分析的40种E.粪便分离物中，9分离物具有HLR至庆大霉素，9分离物具有HLR至链霉素。在39个庆大霉素-HLR肠球菌分离物中，24个分离物是非HLR至链霉素。所有39个与HLR为庆大霉素的分离物以及没有HLR的101个中的19个，携带AAC（6'） - IE-APH（2''） - IA。在31种链霉菌素-HLR分离物中确认Ant（6'） - Ia的托架。 β-内酰胺酶的生产在E.粪便中没有作用于E.粪便和粪便分离株。全基因组测序分析表明，除了携带AAC（6'） - IE-APH（2'） - IA和Ant（6'） - IA的所有粪便分离物 - IA和ant（6'） - IA（n = 8），ST16（n = 4）或ST179（n = 9）。然而，大多数分离物对都有＆即使在相同ST的分离物中也是单核苷酸多态性。

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