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Antimicrobial Peptides Grafted onto a Plasma Polymer Interlayer Platform: Performance upon Extended Bacterial Challenge

机译：接枝到血浆聚合物中间平台上的抗微生物肽：延长细菌挑战时的性能

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摘要

To combat infections on biomedical devices, antimicrobial coatings have attracted considerable attention, including coatings comprising naturally occurring antimicrobial peptides (AMPs). In this study the aim was to explore performance upon extended challenge by bacteria growing in media above samples. The AMPs LL37, Magainin 2, and Parasin 1 were selected on the basis of well-known membrane disruption activity in solution and were covalently grafted onto a plasma polymer platform, which enables application of this multilayer coating strategy to a wide range of biomaterials. Detailed surface analyses were performed to verify the intended outcomes of the coating sequence. Samples were challenged by incubation in bacterial growth media for 5 and 20 h. Compared with the control plasma polymer surface, all three grafted AMP coatings showed considerable reductions in bacterial colonization even at the high bacterial challenge of initial seeding at 1 × 107 CFU, but there were increasing numbers of dead bacteria attached to the surface. All three grafted AMP coatings were found to be non-toxic to primary fibroblasts. These coatings thus could be useful to produce antibacterial surface coatings for biomaterials, though possible consequences arising from the presence of dead bacteria need to be studied further, and compared to non-fouling coatings that avoid attachment of dead bacteria.
机译：为了对生物医学装置的感染，抗微生物涂层引起了相当大的关注，包括涂层，包括包括天然存在的抗微生物肽（AMPS）的涂层。在这项研究中，目的是探讨在样品上方培养基中生长的细菌彻底挑战的表现。在溶液中的众所周知的膜破坏活性的基础上选择AMPS LL37，Magainin 2和寄生虫1，并将其共价接枝到血浆聚合物平台上，这使得这种多层涂层策略能够在广泛的生物材料中施加到各种生物材料上。进行详细的表面分析以验证涂层序列的预期结果。样品通过在细菌生长介质中孵育5和20小时来挑战。与对照等离子体聚合物表面相比，即使在1×107 CFU的初始播种的高细菌挑战中，所有三种接枝的AMP涂层也表现出细菌定植的显着降低，但是越来越多地连接到表面上的死菌数。发现所有三种接枝的AMP涂层对原发性成纤维细胞无毒。因此，这些涂层可用于产生用于生物材料的抗菌表面涂料，但是需要进一步研究死亡细菌的可能性后果，并与避免止死细菌附着的非结垢涂层相比。

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