...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Coatings >Effects of Autologous Bone Marrow Mesenchymal Stem Cells and Platelet-Rich Plasma on Bone Regeneration and Osseointegration of a Hydroxyapatite-Coated Titanium Implant
【24h】

Effects of Autologous Bone Marrow Mesenchymal Stem Cells and Platelet-Rich Plasma on Bone Regeneration and Osseointegration of a Hydroxyapatite-Coated Titanium Implant

机译：自体骨髓间充质干细胞和富含血小板血浆对骨再生骨再生和骨整合的影响

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bone regeneration remains one of the major clinical needs in orthopedics, and advanced and alternative strategies involving bone substitutes, cells, and growth factors (GFs) are mandatory. The purpose of this study was to evaluate whether the association of autologous bone marrow mesenchymal stem cells (BMSC), isolated by ‘one-step surgical procedure’, and activated platelet rich plasma (PRP) improves osseointegration and bone formation of a hydroxyapatite-coated titanium (Ti-HA) implant, already in clinical use, in a rabbit cancellous defect. The GFs present in plasma, in inactivated and activated PRP were also tested. At 2 weeks, histology and histomorphometry highlighted increased bone-to-implant contact (BIC) in Ti-HA combined with BMSC and PRP in comparison to Ti-HA alone and Ti-HA PRP. The combined effect of BMSC and PRP peaked at 4 weeks where the BIC value was higher than all other treatments. At both experimental times, newly formed bone (Trabecular Bone Volume, BV/TV) in all tested treatments showed increased values in comparison to Ti-HA alone. At 4 weeks Ti-HA PRP BMSC showed the highest BV/TV and the highest osteoblasts number; additionally, a higher osteoid surface and bone formation rate were found in Ti-HA BMSC PRP than in all other treatments. Finally, the analyses of GFs revealed higher values in the activated PRP in comparison to plasma and to non-activated PRP. The study suggests that the combination of autologous activated PRP, as a carrier for BMSCs, is a promising regenerative strategy for bone formation, osseointegration, and mineralization of bone implants.
机译：骨再生仍然是骨科的主要临床需求之一，涉及骨替代品，细胞和生长因子（GFS）的先进和替代策略是强制性的。本研究的目的是评估自体骨髓间充质干细胞（BMSC）的关联，分离的“一步手术程序”和活性血小板富血浆（PRP）改善了渗透磷灰石涂层的骨整合和骨形成钛（Ti-HA）植入，已经在临床使用中，在兔子松质缺陷中。还测试了血浆中存在的GFS，灭活和活化的PRP中。在2周，组织学和组织形态形状突出显示Ti-Ha中的骨与植入触点（BIC）与BMSC和PRP相结合，与Ti-Ha单独和Ti-HA PRP相比。 BMSC和PRP在4周达到的效果，其中BIC值高于所有其他治疗。在实验时间，在所有测试处理中的新形成的骨（小梁骨体积，BV / TV）显示出与单独的Ti-Ha相比增加的值。在4周TI-HA PRP BMSC显示出最高的BV / TV和最高的成骨细胞数;另外，在Ti-Ha BMSC PRP中发现了更高的骨质表面和骨形成速率而不是所有其他处理。最后，与血浆和未激活的PRP相比，GFS的分析显示活性PRP中的较高值。该研究表明，作为BMSCs的载体，自体活性PRP的组合是骨植入物的骨形成，骨整合和矿化的有希望的再生策略。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号