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首页> 外文期刊>BMC Genomics >Genome-wide development of insertion-deletion (InDel) markers for Cannabis and its uses in genetic structure analysis of Chinese germplasm and sex-linked marker identification
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Genome-wide development of insertion-deletion (InDel) markers for Cannabis and its uses in genetic structure analysis of Chinese germplasm and sex-linked marker identification

机译：用于大麻插入缺失（Indel）标记的基因组开发及其在遗传结构分析中遗传结构分析的遗传结构分析

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摘要

Cannabis sativa L., a dioecious plant derived from China, demonstrates important medicinal properties and economic value worldwide. Cannabis properties have been usually harnessed depending on the sex of the plant. To analyse the genetic structure of Chinese Cannabis and identify sex-linked makers, genome-wide insertion-deletion (InDel) markers were designed and used. In this study, a genome-wide analysis of insertion-deletion (InDel) polymorphisms was performed based on the recent genome sequences. In total, 47,558 InDels were detected between the two varieties, and the length of InDels ranged from 4?bp to 87?bp. The most common InDels were tetranucleotides, followed by pentanucleotides. Chromosome 5 exhibited the highest number of InDels among the Cannabis chromosomes, while chromosome 10 exhibited the lowest number. Additionally, 31,802 non-redundant InDel markers were designed, and 84 primers evenly distributed in the Cannabis genome were chosen for polymorphism analysis. A total of 38 primers exhibited polymorphisms among three accessions, and of the polymorphism primers, 14 biallelic primers were further used to analyse the genetic structure. A total of 39 fragments were detected, and the PIC value ranged from 0.1209 to 0.6351. According to the InDel markers and the flowering time, the 115 Chinese germplasms were divided into two subgroups, mainly composed of cultivars obtained from the northernmost and southernmost regions, respectively. Additional two markers, “Cs-I1–10” and “Cs-I1–15”, were found to amplify two bands (398?bp and 251?bp; 293?bp and 141?bp) in the male plants, while 389-bp or 293-bp bands were amplified in female plants. Using the two markers, the feminized and dioecious varieties could also be distinguished. Based on the findings obtained herein, we believe that this study will facilitate the genetic improvement and germplasm conservation of Cannabis in China, and the sex-linked InDel markers will provide accurate sex identification strategies for Cannabis breeding and production.
机译：衍生自中国的大麻苜蓿L.，展示了全球的重要药用特性和经济价值。大麻属性通常是根据植物的性别来利用。分析中国大麻的遗传结构，并确定性挂链的制造商，设计并使用了基因组的插入缺失（Indel）标记。在该研究中，基于最近的基因组序列进行插入缺失（Indel）多态性的基因组分析。总共检测到两种品种之间的47,558个诱导，并且诱导的长度为4？BP至87？BP。最常见的吲哚是四核苷酸，其次是戊核苷酸。染色体5在大麻染色体中呈现最多的诱导，而染色体10表现出最低数量。另外，设计了31,802个非冗余诱导标记，选择了在大麻基因组中均匀分布的84个引物，用于多态性分析。共有38个引物在三种探剂中表现出多态性，并且多态性引物，其中14个双曲素引物进一步用于分析遗传结构。总共检测到39个片段，PIC值范围为0.1209至0.6351。根据诱导标记和开花时间，115种中药种分为两个亚组，主要由从最北端和最南端地区获得的品种组成。发现额外的两个标记，“CS-I1-10”和“CS-I1-15”，在雄性植物中放大两个带（398？BP和251磅），而389 -BP或293-BP带在雌性植物中扩增。使用这两个标记，也可以区分女性化和脱型品种。基于本文获得的结果，我们认为本研究将促进中国大麻的遗传改善和种质保护，性别联系的诱导标记将为大麻育种和生产提供准确的性别识别策略。

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