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首页> 外文期刊>Nitrogen >Surface Properties and Adherence of Bradyrhizobium diazoefficiens to Glycine max Roots Are Altered When Grown in Soil Extracted Nutrients
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Surface Properties and Adherence of Bradyrhizobium diazoefficiens to Glycine max Roots Are Altered When Grown in Soil Extracted Nutrients

机译：当在土壤提取的营养成长时，将BradyRhizobium diazoeffiens与甘氨酸最大根的表面性质和粘附发生变化

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Soybean roots are colonized and nodulated by multiple strains of compatible nitrogen-fixing rhizobia primarily belonging to the Genus Bradyrhizobium. Motility towards the root and attachment to root hairs are key determinants of competitive colonization and subsequent nodulation. Bacterial surface properties and motility are known to vary with chemical composition of the culture medium, and root adhesion and nodulation occur in a soil environment rather than laboratory medium. We asked whether the nodulation-promoting factors motility, surface hydrophobicity and surface adhesion of Bradyrhizobium are affected by growth in a soil nutrient environment. B. diazoefficiens USDA 110, 126, 3384, and B. elkanii USDA 26 were grown in mineral salt medium with peptone, yeast extract and arabinose (PSY), and in a soil extracted soluble organic matter (SESOM) medium. Surface hydrophobicity was determined by partitioning into hydrocarbon, motility by transition through soft agar, and surface-exposed saccharides by lectin profiling, followed by biofilm formation and soybean root adhesion capacity of populations. SESOM-grown populations were generally less motile and more hydrophobic. They bound fewer lectins than PSY-grown populations, indicating a simpler surface saccharide profile. SESOM populations of USDA 110 did not form detectable biofilm, but showed increased binding to soy roots. Our results indicate that growth in a soil environment impacts surface properties, motility, and subsequent soy root adhesion propensity. Hence, evaluation of Bradyrhizobium for nodulation efficiency should be performed using soil from the specific field where the soybeans are to be planted, rather than laboratory culture media.
机译：大豆根部由主要属于Bradyrhizobium属的多种相容的氮气固定根瘤菌菌株进行殖民和染色。对根部毛的根本和附着的动力是竞争性定植和随后的染色的关键决定因素。已知细菌表面性质和动力与培养基的化学组成变化，并且在土壤环境中而不是实验室培养基中发生根粘附和染色。我们询问促进促进促进因子的动力，表面疏水性和Bradyrohizobium的表面粘附受土壤营养环境的生长影响。 B. Diazoefficiens USDA 110,126,3384和B.Elkanii USDA 26在矿物盐培养基中生长在蛋白胨，酵母提取物和阿拉伯糖（Psy），并在土壤中提取溶于有机物（SESOM）培养基。通过将烃，通过软琼脂的过渡，通过凝集素分析的表面暴露的糖分来确定表面疏水性，然后通过凝集素分析，然后进行生物膜形成和群体的大豆根粘附容量。 SESOM-生长的种群通常不那么易用，更疏水。它们比Psy种群的凝集素少得多，表明表面糖型较差。 USDA 110的SESOM群体没有形成可检测的生物膜，但显示与大豆根的结合增加。我们的结果表明，土壤环境中的生长会影响表面性质，运动和随后的大豆根粘附倾向。因此，应使用来自待种植大豆的特定领域的土壤来进行对结核效率的Bradyrhizobium的评估，而不是实验室培养基。

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