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Studies on Application of Ion Beam Breeding to Industrial Microorganisms at TIARA

机译：离子束育种在粒地区工业微生物中的应用研究

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Mutation-breeding technologies are useful tools for the development of new biological resources in plants and microorganisms. In Takasaki Ion Accelerators for Advanced Radiation Application (TIARA) at the National Institutes for Quantum and Radiological Science and Technology, Japan, ion beams were explored as novel mutagens. The mutagenic effects of various ion beams on eukaryotic and prokaryotic microorganisms were described and their application in breeding technology for industrial microorganisms were discussed. Generally, the relative biological effectiveness (RBE) depended on the liner energy transfer (LET) and the highest RBE values were obtained with 12C5+ ion beams. The highest mutation frequencies were obtained at radiation doses that gave 1%–10% of surviving fraction. By using 12C5+ ion beams in this dose range, many microorganisms have been improved successfully at TIARA. Therefore, ion-beam breeding technology for microorganisms will have applications in many industries, including stable food production, sustainable agriculture, environmental conservation, and development of energy resources in the near future. Moreover, genome analyses of the ion-beam-induced mutants are in progress to clear the differences of mutational functions induced by different LET radiations in microorganisms. Further characterizations of mutations induced by different LET radiations will facilitate more effective use of ion beams in microorganisms breeding.
机译：突变育种技术是在植物和微生物中开发新的生物资源的有用工具。在TAKASAKI离子加速器中，用于国立大量辐射应用（TIARA）的量子，日本，离子梁被探索为新型诱变。讨论了各种离子束对真核和原核微生物对真核和原核微生物的诱变效应，并讨论了它们在工业微生物育种技术中的应用。通常，用12C5 +离子束获得依赖于衬里能量转移（假）和最高RBE值的相对生物效果（RBE）。在辐射剂量下获得最高突变频率，所述辐射剂量得到1％-10％存活级分。通过在该剂量范围内使用12C5 +离子束，在丽拉拉成功地改善了许多微生物。因此，用于微生物的离子束育种技术将在许多行业中具有应用，包括稳定的食品生产，可持续农业，环境保护以及在不久的未来能源的发展。此外，离子束诱导突变体的基因组分析正在进行，以清除不同的突变函数诱导的突变功能的差异，使微生物中的辐射。通过不同的诱导诱导的突变的进一步表征辐射将促进微生物育种中的离子束更有效地使用离子束。

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