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Optical Absorption Cross-Section of DNA Bases—Thymine and Guanine—in the Energy Region from 3.1 to 250 eV (5–400 nm)

机译:DNA碱基和鸟嘌呤的光学吸收横截面从3.1到250eV(5-400nm)的能量区域

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(1) Background: Optical absorption cross-section—the absolute absorption intensity specific to each molecule—of nucleic acid bases enables us to estimate the reaction yields of DNA lesions induced by the exposure to not only photons but also ionizing radiations. However, it was unknown in the energy region exceeding ~10 eV (wavelength ~120 nm). (2) Methods: Thin films of DNA bases—thymine and guanine—were prepared using a vacuum sublimation technique. Absorption spectra of these films were measured in the energy region from 3.1 to 250 eV (5–400 nm) at the synchrotron radiation facility UVSOR. (3) Results: The absorption spectra of both bases exhibited prominent absorption peaks around 20 eV and smaller peaks in the energy region below 10 eV. The determined optical oscillator strength distribution was verified to be reasonable based on the Thomas–Reiche–Kuhn oscillator strength sum rule. (4) Conclusion: Most of the oscillator strength distribution was positioned in the measured energy region, and therefore the absorption spectra significantly contributed to the quantitative study for the photo and radiation-chemical reactions of DNA.
机译:(1)背景:光学吸收横截面 - 对每个分子核酸碱基的绝对吸收强度使我们能够估计暴露于光子的暴露而且也是电离辐射诱导的DNA病变的反应产率。然而,在超过〜10eV的能量区域中未知(波长和120nm)。 (2)方法:使用真空升华技术制备DNA碱基和鸟嘌呤的薄膜。在同步辐射设施UVSOR的3.1至250eV(5-400nm)的能量区域中测量这些薄膜的吸收光谱。 (3)结果:两种碱基的吸收光谱在10eV低于10eV的能量区域中呈现突出的吸收峰和较小的峰。基于Thomas-Reiche-Kuhn振荡器强度规则,验证了确定的光学振荡器强度分布是合理的。 (4)结论:大多数振荡器强度分布定位在测量的能量区域中,因此吸收光谱显着促进了DNA照片和辐射 - 化学反应的定量研究。

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