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首页> 外文期刊>Doklady biochemistry & biophysics >A New Method of Deleting a Specified Sequence in Transgenic Lines of Drosophila melanogaster
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A New Method of Deleting a Specified Sequence in Transgenic Lines of Drosophila melanogaster

机译：删除果蝇转基因品系中特定序列的新方法

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Today, the regulation of gene expression in higher eukaryotes is investigated at a new level: instead of studying the activity of individual enhancer or promoters, complex regulatory systems consisting of several components are studied. Due to the effect of position of a genetic construct in the genome, the expression of genes of the same genetic construct may considerably differ in different transgenic lines. This phenomenon may be caused by the regulatory elements flanking the site of insertion of the construct into the genome. Therefore, when studying the interaction between the regulatory elements, it is necessary to have an opportunity to eliminate each of these elements from genetic constructs. For this purpose, the Cre/loxP and FLP/FRT recombinase systems are used, which allow part of constructs to be eliminated from the genome of transgenic flies. However, the two systems are not sufficient for performing many modern studies. For example, to study the interaction between insulators, it is necessary to excise all (at least two) insulators and enhancers of marker genes of the construct.
机译：今天，对高等真核生物中基因表达的调控已在一个新的水平上进行了研究：不是研究单个增强子或启动子的活性，而是研究由几种成分组成的复杂调控系统。由于基因构建体在基因组中的位置的影响，同一基因构建体的基因表达在不同的转基因品系中可能有很大差异。这种现象可能是由于构建体插入基因组的插入位点两侧的调控元件引起的。因此，在研究调节元件之间的相互作用时，有必要从遗传构建体中消除这些元件中的每一个。为此目的，使用Cre / loxP和FLP / FRT重组酶系统，其允许从转基因果蝇的基因组中消除部分构建体。但是，这两个系统不足以进行许多现代研究。例如，为了研究绝缘子之间的相互作用，有必要切除所有（至少两个）绝缘子和构建体标记基因的增强子。

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