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首页> 外文期刊>Ecotoxicology and Environmental Safety >Reliability of plant root comet assay in comparison with human leukocyte comet assay for assessment environmental genotoxic agents
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Reliability of plant root comet assay in comparison with human leukocyte comet assay for assessment environmental genotoxic agents

机译：植物根彗星试验与人白细​​胞彗星试验相比可评估环境遗传毒性剂的可靠性

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Comet assay is an efficient test to detect genotoxic compounds based on observation of DNA damage. The aim of this work was to compare the results obtained from the comet assay in two different type of cells extracted from the root tips from Lactuca saliva L. and human blood. For this, Spent Pot Liner (SPL), and its components (aluminum and fluoride) were applied as toxic agents. SPL is a solid waste generated in industry from the aluminum mining and processing with known toxicity. Three concentrations of all tested solutions were applied and the damages observed were compared to negative and positive controls. It was observed an increase in the frequency of DNA damage for human leukocytes and plant cells, in all treatments. On human leukocytes, SPL induced the highest percentage of damage, with an average of 87.68%. For root tips cells of L. saliva the highest percentage of damage was detected for aluminum (93.89%). Considering the arbitrary units (AU), the average of nuclei with high levels of DNA fragmentation was significant for both cells type evaluated. The tested cells demonstrated equal effectiveness for detection of the genotoxicity induced by the SPL and its chemical components, aluminum and fluoride. Further, using a unique method, the comet assay, we proved that cells from root tips of Lactuca saliva represent a reliable model to detect DNA damage induced by genotoxic pollutants is in agreement of those observed in human leukocytes as model. So far, plant cells may be suggested as important system to assess the toxicological risk of environmental agents.
机译：彗星分析是一种基于DNA损伤观察来检测遗传毒性化合物的有效测试。这项工作的目的是比较从彗星唾液和人血的根尖提取的两种不同类型细胞中的彗星测定结果。为此，将废锅衬里（SPL）及其成分（铝和氟化物）用作有毒物质。 SPL是工业上从铝的开采和加工过程中产生的具有已知毒性的固体废物。施加所有测试溶液的三种浓度，并将观察到的损伤与阴性和阳性对照进行比较。在所有处理中，都观察到对人类白细胞和植物细胞的DNA损伤频率增加。在人白细胞上，SPL引起的损伤百分比最高，平均为87.68％。对于唾液乳杆菌的根尖细胞，铝的检出率最高（93.89％）。考虑到任意单位（AU），对于两种评估的细胞类型，具有高水平DNA片段化的细胞核的平均值都很重要。测试的细胞在检测SPL及其化学成分铝和氟化物引起的基因毒性方面显示出同等效力。此外，使用一种独特的方法，彗星试验，我们证明了来自唾液乳杆菌根尖的细胞代表了一种可靠的模型，可以检测由遗传毒性污染物引起的DNA损伤，与在人类白细胞模型中观察到的一致。迄今为止，植物细胞可能被建议作为评估环境因素毒理学风险的重要系统。

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