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首页> 外文期刊>Ecotoxicology and Environmental Safety >Effects of chronic exposures to the herbicides atrazine and glyphosate to larvae of the threespine stickleback (Gasterosteus aculeatus)
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Effects of chronic exposures to the herbicides atrazine and glyphosate to larvae of the threespine stickleback (Gasterosteus aculeatus)

机译：长期暴露于除草剂阿特拉津和草甘膦对三脊棘背le（Gasterosteus aculeatus）幼虫的影响

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摘要

Atrazine (ATR) and glyphosate (GLY) are among the most widely used herbicides in Canada, yet there is relatively little information concerning their toxicity to early life stages of marine fish. The threespine stickleback (Gasterosteus aculeatus) reproduces in coastal habitats which receive runoff of pesticides during the summer, the peak season of herbicide use. Sticklebacks have biomarkers for effects of both estrogenic and androgenic contaminants. Stickleback adults from a clean reference site were allowed to reproduce in the laboratory and the fertilized eggs were incubated until hatching. Larval sticklebacks ( < 24 h old) were exposed for 42d to four concentrations (0.1,1,10 and 100 μg/l) of either ATR or GLY, a seawater control, a carrier (acetone) control and positive controls for estrogenic (0.05 μg/l ethinylestradiol, EE2) and androgenic (3 μg/l dihydrotestosterone, DHT) effects. The survivors were measured (length, wet weight) then conserved for biochemical (vitellogenin, VTG, and the male nest-protein spiggin, SPG) and histological (phenotypic sex determination) analyses. There were no significant effects of ATR and GLY exposures on larval survival or growth. Exposure to 3 μg DHT/l resulted in a significant effect on growth (body lengths) but did not induce SPG, possibly because of DHT degradation after the 24 h solution renewal. VTG was induced after the EE2 exposure, yet neither ATR nor GLY induced production of VTG and SPG. The proportion of mixed sex individuals was higher in the positive controls compared to the negative controls. A single mixed sex individual was found in the group exposed to the lowest dose of atrazine and none in glyphosate expositions. We conclude that these herbicides do not show estrogenic or androgenic effects to early life stages of sticklebacks at environmentally realistic concentrations.
机译：阿特拉津（ATR）和草甘膦（GLY）是加拿大使用最广泛的除草剂，但关于其对海水鱼类早期生命期的毒性的信息相对较少。三脊棘背le（Gasterosteus aculeatus）繁殖于沿海生境，在夏季（除草剂使用的高峰季节）接受农药径流。棘背动物具有生物标记物，可同时检测雌激素和雄激素污染物。允许来自干净参考地点的背成虫在实验室中繁殖，然后将受精卵孵化直至孵化。将幼体stick（<24小时龄）暴露于42种浓度的ATR或GLY，海水对照，载体（丙酮）对照和雌激素阳性对照（0.05的四个浓度（0.1、1、10和100μg/ l）中42d）微克/升乙炔雌二醇，EE2）和雄激素（3微克/升二氢睾丸激素，DHT）作用。测量幸存者（长度，湿重），然后保存以进行生化分析（玻璃纤维蛋白原，VTG，雄性巢蛋白菌，SPG）和组织学分析（表型性别确定）。 ATR和GLY暴露对幼虫存活或生长没有显着影响。暴露于3μgDHT / l会对生长（体长）产生显着影响，但不会诱导SPG，这可能是由于24 h溶液更新后DHT降解所致。在暴露EE2之后诱导了VTG，但是ATR和GLY均未诱导VTG和SPG的产生。与阴性对照相比，阳性对照中混合性别个体的比例更高。在暴露于最低剂量的阿特拉津的组中发现了一个单一的混合性个体，而在草甘膦暴露中则没有。我们得出的结论是，在环境实际浓度下，这些除草剂对stick的早期生命阶段没有显示雌激素或雄激素作用。

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  • 来源
    《Ecotoxicology and Environmental Safety》 |2013年第1期|174-181|共8页
  • 作者

    Charline Le Mer; Robert L. Roy; Jocelyne Pellerin; Catherine M. Couillard; Domynick Maltais;

  • 作者单位

    ISMER, Universite du Quebec a Rimouski, 300 Allee des Ursulines, P.O. Box 3300, Rimouski, Quebec, Canada C5L 3A1;

    Department of Fisheries and Oceans, Maurice Lamontagne Institute, P.O. Box WOO, 850 route de la mer, Mont-Joli, Quebec, Canada C5H 3Z4;

    ISMER, Universite du Quebec a Rimouski, 300 Allee des Ursulines, P.O. Box 3300, Rimouski, Quebec, Canada C5L 3A1;

    Department of Fisheries and Oceans, Maurice Lamontagne Institute, P.O. Box WOO, 850 route de la mer, Mont-Joli, Quebec, Canada C5H 3Z4;

    Department of Fisheries and Oceans, Maurice Lamontagne Institute, P.O. Box WOO, 850 route de la mer, Mont-Joli, Quebec, Canada C5H 3Z4;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    gasterosteus aculeatus; larval bioassay; atrazine; glyphosate; vitellogenin; spiggin; ELISA; ovotestis;

    机译：腓肠肌aculeatus;幼虫生物测定阿特拉津草甘膦卵黄蛋白原斯皮金ELISA;卵睾丸;

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