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首页> 外文期刊>Energy Conversion & Management >Response surface optimization of enzymatic hydrolysis of narrow-leaf cattail for bioethanol production
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Response surface optimization of enzymatic hydrolysis of narrow-leaf cattail for bioethanol production

机译：窄叶香蒲酶解生产生物乙醇的响应面优化

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Narrow-leaf cattail was employed as lignocellulosic biomass substrate for the investigation of the hydrolysis process of lignocellulosic ethanol. Cellulose saccharification into a high yield of fermentable sugar is an important step in ethanol production. Response surface methodology was utilized in the study of variables affecting enzymatic hydrolysis on the released glucose and xylose. Five levels (-2, -1, 0, +1, +2) of independent variable factors; cellulase (5-25 FPU/g substrate), p-glucosidase (0-20 U/g substrate), hydrolysis temperature (30-50 ℃), and hydrolysis time (24-96 h), were randomly setup by using the Design of Experiment program. The significance of the regression model was high; with 95% confidence interval (less than 5% error). The predicted result after optimization was also in good agreement with the experimental data. An optimal condition; 13.50 FPU/g substrate, 16.50 U/g substrate, 50 ℃ and 24 h, was obtained, yielding a released glucose of 552.9 mg/g substrate (75.6% saccharification) and a released xylose of 74.0 mg/g substrate (45.6% saccharification).
机译：窄叶香蒲被用作木质纤维素生物质的底物，用于研究木质纤维素乙醇的水解过程。将纤维素糖化为高产可发酵糖是乙醇生产中的重要步骤。响应面法被用于研究影响释放的葡萄糖和木糖上酶促水解的变量。五级（-2，-1、0，+ 1，+ 2）自变量因子；通过使用Design随机设置纤维素酶（5-25 FPU / g底物），对葡萄糖苷酶（0-20 U / g底物），水解温度（30-50℃）和水解时间（24-96 h）。实验程序。回归模型的意义很高。置信区间为95％（误差小于5％）。优化后的预测结果也与实验数据吻合良好。最佳条件；获得50.℃和24 h的13.50 FPU / g底物，16.50 U / g底物，释放的葡萄糖为552.9 mg / g底物（糖化率为75.6％）和释放的木糖为74.0 mg / g底物（糖化率为45.6％）。 ）。

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