Intracellular Forms of Adenovirus DNA II. Isolation in Dye-Buoyant Density Gradients of a DNA-RNA Complex from KB Cells Infected with Adenovirus Type 2

Walter Doerfler; Ulla Lundholm; Ute Rensing; Lennart Philipson

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Intracellular Forms of Adenovirus DNA II. Isolation in Dye-Buoyant Density Gradients of a DNA-RNA Complex from KB Cells Infected with Adenovirus Type 2

机译：细胞内形式的腺病毒DNA II。用腺病毒2型感染的KB细胞分离DNA-RNA复合物的染料浮力密度梯度

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DNA-RNA complexes with different DNA/RNA ratios have been isolated from KB cells productively infected with human adenovirus type 2 by the dye-buoyant density procedure by using propidium iodide. Both the DNA and RNA components of these complexes are virus specific, and parental as well as newly synthesized viral DNA can be recovered from these complexes. The RNA component is susceptible to digestion with pancreatic ribonuclease at low and high salt concentrations. The RNA is in part liberated from the complex during purification over several cycles of equilibrium centrifugation in dye-buoyant density and Cs₂SO₄ gradients. Analysis in the latter gradients reveals at least four classes of virus-specific nucleic acid: (i) RNA loosely bound and released during purification, (ii) and (iii) two distinct classes of RNA-DNA complexes with different DNA/RNA ratios, and (iv) DNA apparently not associated with RNA. The size of the RNA molecules is large but varying in the different classes of complexes. The biological function of these complexes is still uncertain, but they may be transcription complexes.

机译：通过使用碘化丙烯酸盐，通过用染料浮力密度方法从KB细胞中分离出具有不同DNA / RNA比率的DNA-RNA复合物。这些配合物的DNA和RNA组分都是特异性的病毒，并且可以从这些配合物中回收父母以及新合成的病毒DNA。 RNA组分易于在低盐核糖酶的用胰腺核糖核酸酶消化。在纯化期间，RNA部分从复合物中释放出几个平衡离心染料浮力密度和Cs _{2 SO _{4 梯度。后梯度的分析显示至少四种病毒特异性核酸：（i）在纯化期间松散地结合和释放的RNA，（ii）和（iii）具有不同DNA / RNA比例的两个不同类别的RNA-DNA复合物，（iv）DNA显然与RNA无关。 RNA分子的尺寸大，但在不同类别的复合物中变化。这些配合物的生物学功能仍然不确定，但它们可能是转录复合物。}}

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《Journal of Virology》 |1973年第4期|共15页
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Walter Doerfler; Ulla Lundholm; Ute Rensing; Lennart Philipson;
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1. Intracellular Forms of Adenovirus DNA II. Isolation in Dye-Buoyant Density Gradients of a DNA-RNA Complex from KB Cells Infected with Adenovirus Type 2 [J] . Walter Doerfler, Ulla Lundholm, Ute Rensing, Journal of Virology . 1973,第4期

机译：细胞内形式的腺病毒DNA II。用腺病毒2型感染的KB细胞分离DNA-RNA复合物的染料浮力密度梯度
2. Intracellular Forms of Adenovirus DNA II. Isolation in Dye-Buoyant Density Gradients of a DNA-RNA Complex from KB Cells Infected with Adenovirus Type 2 [J] . Walter Doerfler, Ulla Lundholm, Ute Rensing, Journal of Virology . 1973,第4期

机译：细胞内形式的腺病毒DNA II。用腺病毒2型感染的KB细胞分离DNA-RNA复合物的染料浮力密度梯度
3. Intracellular Forms of Adenovirus Deoxyribonucleic Acid I. Evidence for a Deoxyribonucleic Acid-Protein Complex in Baby Hamster Kidney Cells Infected with Adenovirus Type 12 [J] . Walter Doerfler, Ulla Lundholm, Monica Hirsch-Kauffmann Journal of Virology . 1972,第2期

机译：腺病毒脱氧核糖核酸的细胞内形式。婴儿仓鼠肾细胞中脱氧核糖核酸蛋白复合物的证据12型感染腺病毒12型
4. Real-Time Intracellular Transport and Trafficking of Adenovirus/PEI/DNA Nanocomplexes in Live HeLa Cells [C] . Eric Jabart, Yoojin An, Samuel Lai, Annual meeting exposition of the Controlled Release Society . 2005

机译：实时HeLa细胞中腺病毒/ PEI / DNA纳米复合物的实时细胞内运输和运输
5. Methylation and chromatin conformation of adenovirus type 12 DNA sequences in transformed cells. [D] . Redfield, Rosemary Jeanne. 1980

机译：转化细胞中12型腺病毒DNA序列的甲基化和染色质构象。
6. Intracellular Forms of Adenovirus DNA II. Isolation in Dye-Buoyant Density Gradients of a DNA-RNA Complex from KB Cells Infected with Adenovirus Type 2 [O] . Walter Doerfler, Ulla Lundholm, Ute Rensing, 1973

机译：腺病毒DNA II的细胞内形式。从感染了2型腺病毒的KB细胞中的DNA-RNA复合物的染料-浮力密度梯度中分离
7. Intracellular Forms of Adenovirus DNA III. Integration of the DNA of Adenovirus Type 2 into Host DNA in Productively Infected Cells [O] . Harold Burger, Walter Doerfler 1974

机译：细胞内形式的腺病毒DNA III。将腺病毒2型DNA的整合到高产细胞中的宿主DNA中

Intracellular Forms of Adenovirus DNA II. Isolation in Dye-Buoyant Density Gradients of a DNA-RNA Complex from KB Cells Infected with Adenovirus Type 2

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