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首页> 外文期刊>Journal of Virology >Persistence of the Viral Genome in Adenovirus Type 12-infected Hamster Cells
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Persistence of the Viral Genome in Adenovirus Type 12-infected Hamster Cells

机译：腺病毒类型12感染的仓鼠细胞中病毒基因组的持久性

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摘要

Induction of T antigen by adenovirus type 12 was studied in growing and growth-inhibited cultures of the Nil-2 line of Syrian hamster cells. At a viral input multiplicity of 10, neoantigen was present in 100% of the cells by 24 hr. T antigen gradually disappeared in descendants of these cells so that 2 weeks after infection only 1% gave specific immunofluorescence. When cellular replication was prevented by addition of fluorodeoxyuridine, T antigen persisted in all cells for the 2-week period. Upon infection of growing cultures with purified 3H-labeled adenovirus type 12 and autoradiographic analysis of the cells at various times thereafter, a gradual reduction in labeled nuclear loci was noted which paralleled the decrease in T antigen-containing cells. In nongrowing cultures, no change in labeled loci was noted. Correlation of T antigen and labeled loci revealed that fluorescent cells contained, on the average, about 10 times more silver grains than nonfluorescent cells. All of 92 preselected fluorescent cells showed labeled loci, whereas, of 100 nonfluorescent cells, 18 were free of silver grains. The implications of these findings are discussed.
机译：在叙利亚仓鼠细胞的尼尔-2系列的生长和生长抑制培养中，研究了腺病毒12型腺病毒12系的诱导。在病毒输入多重10中，Neoantigen在100％的细胞中以24小时以100％存在。 T抗原在这些细胞的后代逐渐消失，使感染后2周仅为1％给出特定的免疫荧光。当通过加入氟脱氧核素预防细胞复制时，在所有细胞中持续到2周的T抗原。在用纯化的 3 H标记的腺病毒型12型生长培养物中，并在不同时间对细胞进行放射显影分析，并注意到标记的核基因座的逐渐减少，其平行于含抗原的降低细胞。在非研磨培养中，注意到标记的基因座没有变化。 T抗原和标记基因座的相关性显示荧光细胞含有比非荧光细胞的约10倍的银粒。所有92个预选的荧光细胞显示出标记的基因座，而100个非荧光细胞，18个不含银颗粒。讨论了这些发现的含义。

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