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An Analysis of the Vi-Phage Typing Scheme for Salmonella typhi

机译:Salmonella Typhi的VI-噬菌体键入方案分析

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SUMMARY: The seventy-two currently recognized Vi-phage types of Salmonella typhi have been examined in order to determine, where possible, their structural formulae. The genetic nature of the changes in host range of the corresponding typing adaptations of the Vi-phage II has also been investigated.Thirty-seven temperate phages have been isolated from the type strains and type-determining ability has been demonstrated in all but five of these phages. Several of the determining phages were found in more than one type strain and it is probable that no more than 16 different determining phages are represented in our collection. Because there are fewer phage reactions to be eliminated in cultures already possessing a restricted sensitivity spectrum to the typing adaptations of Vi-phage II, some of the determining phages (‘semi-determining phages’) display their full effect only in such cultures.One determining phage, d1-C, can co-operate with others, especially phages d8 and d9, in multiply lysogenized cultures to produce an effect which is more than a summation of the known type-determining powers of the individual phages concerned. Moreover, the type-determining effects of phages d1-C, d8, and d9 in singly or doubly lysogenized cultures are influenced by the temperature of incubation.As has been shown by earlier workers, the changes in host-range of Vi-phage II that produce the Vi-typing phages may be phenotpic (host-induced) or genotypic (mutational) in nature, or a combination of these two types of variation. Amongst the current typing phages are represented eighteen different phenotypic modifications and forty-three different host-range mutants. Many of these mutations seem to be related to each other but their chromosomal distribution has not been mapped.
机译:发明内容:研究了72个目前公认的Salmonella Typhi的VI-Phiage类型,以便在可能的情况下确定它们的结构性公式。还研究了VI-phage II的相应键入适应的宿主范围的变化的遗传性质。已经从菌株类型中分离出四节的温带噬菌体,并且在除其中的五个中已经证明了型菌株这些噬菌体。在多于一种类型的菌株中发现了几个测定噬菌体,并且可能在我们的收集中表示不超过16个不同的测定噬菌体。因为在已经具有限制敏感谱的培养物中消除了较少的噬菌体反应,以便键入的vi-phage II的打字适应,一些确定噬菌体('半确定噬菌体')仅在这种培养物中显示它们的全部效果。确定噬菌体D1-C,可以与其他型培养物中的其他卵泡D8和D9共同运行,以产生效果,其大于所涉及各个噬菌体的已知类型测定功率的求和。此外,噬菌体D1-C,D8和D9在单独或双溶酶化培养物中的类型测定效果受孵育温度的影响。已被早期工人所示,VI-Phage II的主体范围的变化产生VI键入的噬菌体可以是自然界中的诸如诸如这两种类型的变异的组合的均等(宿主诱导的)或基因型(突变)。在目前的键入噬菌体中表示为18个不同的表型修饰和四十三种不同的宿主范围突变体。许多这些突变似乎彼此相关,但它们的染色体分布尚未被映射。

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