Evidence for involvement of an RNA primer in initiation of strong-stop plus DNA synthesis during reverse transcription in vitro.

C A Omer; R Resnick; A J Faras

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Evidence for involvement of an RNA primer in initiation of strong-stop plus DNA synthesis during reverse transcription in vitro.

机译：RNA引物参与RNA引物在体外反转转录期间对逆转转录的强度加DNA合成开始的证据。

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Employing enzymatic reactions in vitro, we have identified the presence of oligoribonucleotides at the 5' end of strong-stop plus [(+)] DNA. Similar results were obtained whether the strong-stop (+) DNA was synthesized by preparations of detergent-disrupted avian sarcoma virus or reconstructed reactions containing purified reverse transcriptase and a template that mimics the purported natural template for strong-stop (+) DNA synthesis. The latter reactions provide a system to delineate more precisely the discrete requirements necessary for the initiation and synthesis of this species of (+) DNA.

机译：在体外使用酶促反应，我们已经确定了在强 - 止动加（+）] DNA的5'末端在5'末端存在寡核苷酸的存在。是否通过制剂通过洗涤剂破坏的禽类病毒或含有纯化的逆转录酶的重建反应和模板来合成了相似的结果和模拟了用于强度（+）DNA合成的典型的自然模板的模板。后一种反应提供了一种更精确地描绘的系统，该系统可以更精确地绘制，所以（+）DNA的启动和合成所需的离散要求。

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《Journal of Virology》 |1984年第2期|共6页
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C A Omer; R Resnick; A J Faras;
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1. Evidence for involvement of an RNA primer in initiation of strong-stop plus DNA synthesis during reverse transcription in vitro. [J] . C A Omer, R Resnick, A J Faras Journal of Virology . 1984,第2期

机译：RNA引物参与RNA引物在体外反转转录期间对逆转转录的强度加DNA合成开始的证据。
2. Evidence for involvement of an RNA primer in initiation of strong-stop plus DNA synthesis during reverse transcription in vitro. [J] . C A Omer, R Resnick, A J Faras Journal of Virology . 1984,第2期

机译：RNA引物参与RNA引物在体外反转转录期间对逆转转录的强度加DNA合成开始的证据。
3. DE NOVO AND DNA PRIMER-MEDIATED INITIATION OF CDNA SYNTHESIS BY THE MAURICEVILLE RETROPLASMID REVERSE TRANSCRIPTASE INVOLVE RECOGNITION OF A 3' CCA SEQUENCE [J] . Chen B., Lambowitz AM. Journal of Molecular Biology . 1997,第3期

机译：从头开始，DNA底物介导的莫里斯维尔逆转录酶逆转录酶涉及3'CCA序列的识别
4. A Mathematical Proof of Double Helix DNA to Reverse Transcription RNA for Bioinformatics [C] . Moon Ho Lee, Han Hai, Sung Kook Lee, International Conference of Artificial Intelligence, Medical Engineering, Education . 2017

机译：双螺旋DNA对逆转录RNA进行生物信息学的数学证明
5. Coupling selection of the HIV-1 tRNA primer used for reverse transcription with viral translation and encapsidation [D] . Djekic, Uros V. 2007

机译：用于逆转录的HIV-1 tRNA引物与病毒翻译和衣壳化的偶联选择
6. Evidence for involvement of an RNA primer in initiation of strong-stop plus DNA synthesis during reverse transcription in vitro. [O] . C A Omer, R Resnick, A J Faras 1984

机译：在体外反转录过程中RNA引物参与强终止和DNA合成启动的证据。
7. Evidence for circularization of the avian oncornavirus RNA genome during proviral DNA synthesis from studies of reverse transcription in vitro. [O] . Collett, M S, Faras, A J 1976

机译：根据体外反转录研究，在原病毒DNA合成过程中禽流感病毒基因组环化的证据。

Evidence for involvement of an RNA primer in initiation of strong-stop plus DNA synthesis during reverse transcription in vitro.

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