• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Transcriptional and posttranscriptional control of c-myc gene expression in WEHI 231 cells.
【24h】

Transcriptional and posttranscriptional control of c-myc gene expression in WEHI 231 cells.

机译：魏氏231细胞C-MYC基因表达的转录和前术治疗。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Incubation of WEHI 231 cells, derived from a murine B-cell lymphoma, with antisera directed against its surface immunoglobulin results in the inhibition of growth within 24 h. Previously, we demonstrated that this treatment selectively affects cytoplasmic levels of c-myc mRNA (J. E. McCormack, V. H. Pepe, R. B. Kent, M. Dean, A. Marshak-Rothstein, and G. E. Sonenshein, Proc. Natl. Acad. Sci. USA 81:5546-5550, 1984). An initial increase in the cytoplasmic mRNA level is followed by a precipitous drop. We now show that the early increase results from a dramatic increase in the rate of c-myc gene transcription, as well as from partial stabilization of the mRNA in the cytoplasm. The later decrease results from a shutdown in transcription of the c-myc gene and a return to the normal lability of the cytoplasmic c-myc mRNA. Treatment with phorbol ester, like treatment with anti-immunoglobulin sera, inhibited WEHI 231 cell growth and caused similar changes in cytoplasmic c-myc mRNA levels, which can also be related to alterations in c-myc gene transcription. These results indicate that the control of c-myc gene expression in B cells is effected through regulation at multiple levels.
机译：培养来自鼠b细胞淋巴瘤的韦赫231细胞，抗血清针对其表面免疫球蛋白导致24小时内的生长抑制。以前，我们证明这种治疗选择性地影响C-Myc mRNA的细胞质水平（JE McCormack，VH Pepe，RB Kent，M. Dean，A.Marshak-Rothstein，以及Ge Sonenhein，Proc。Natl。Acad。SCI。SCI。美国81 ：5546-5550,1984）。细胞质mRNA水平的初始增加之后是陡峭的下降。我们现在表明早期增加了C-Myc基因转录速率的显着增加，以及细胞质中mRNA的部分稳定性。后来降低了C-MYC基因转录的关断和恢复到细胞质C-MYC mRNA的正常易易损伤的结果。用抗免疫球蛋白血清处理的Phorbol酯治疗抑制韦氏231细胞生长，并导致细胞质C-Myc mRNA水平的相似变化，也可以与C-Myc基因转录的改变有关。这些结果表明，通过多水平的调节来实现B细胞中的C-Myc基因表达的控制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号