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首页> 外文期刊>Molecular and Cellular Biology >Expression of the intracisternal A-particle is elevated during differentiation of embryonal carcinoma cells.
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Expression of the intracisternal A-particle is elevated during differentiation of embryonal carcinoma cells.

机译：在胚胎癌细胞的分化期间升高了脑内α-颗粒的表达。

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Three cDNA clones coding for the 3' region of the intracisternal A-particle (IAP), a mouse endogenous retrovirus, were isolated during screening of a library for genes whose expression was modulated during the retinoic acid-induced differentiation of the embryonal carcinoma cell line F9 into parietal endoderm-like (PE-like) cells. In contrast to previously reported results, no IAP transcripts were detected in either F9 cells or two pluripotent cell lines tested. Instead, IAP transcripts as well as IAPs were abundant in the PE-like cells PYS-2 and F9AcCl 9 and in retinoic acid-induced F9 cells but not in the other differentiated cell types of teratocarcinoma origin which were examined. A comparison of the nucleotide sequences of the three IAP cDNA clones with a genomically integrated proviral sequence (MIA14) demonstrated heterogeneity in both length and sequence among the clones. The position of the poly(A) addition site was determined to be 15 nucleotides from the proposed poly(A) addition signal and to occur after the sequence CAGA, not CA, as previously proposed. Length heterogeneity was greatest in a region of TC repeats 80 base pairs 5' to the poly(A) addition site. Additionally, the putative TATAA box found in MIA14 was deleted in the cDNA clones and in the long terminal repeat regions from two other genomic clones examined. The heterogeneity evident among the cDNA clones further demonstrated that at least two distinct IAP genes are activated during differentiation. An analysis of the rate of transcription in isolated nuclei indicated that the activation of expression of IAP genes in PE-like cells is the result of transcriptional regulation. Together, these observations suggest that the modulation of IAP transcription is regulated autonomously rather than by the fortuitous integration of an IAP sequence adjacent to a developmentally regulated cellular gene.
机译：在筛选中，分离了对脑内α-粒子（IAP）的3'区域的三个cDNA克隆，在胚胎癌细胞诱导的视黄酸诱导的分化期间调节表达的基因文库期间F9进入顶胚层（PE样）细胞。与先前报道的结果相比，在F9细胞或测试的两个多能细胞系中检测到IAP转录物。相反，IAP转录物以及IAPS在PE样细胞Pys-2和F9AcCl 9以及维甲酸诱导的F9细胞中，但不在检查的其他分化细胞类型中进行检查。三种IAP cDNA克隆核苷酸序列与基因组综合的荧光序列（MIA14）的比较，在克隆中的长度和序列中显示出异质性。来自所提出的聚（a）添加信号的聚（a）加成部位的位置是15个核苷酸，并且在序列Caga，而不是先前提出的情况下发生。在TC的区域中，在TC的区域中最大的长度是最大的，在Poly（a）加成位点重复80个碱基对5'。另外，在CDNA克隆中删除MIA14中发现的推定的TATA盒，并从检查的另外两种基因组克隆中删除在cDNA克隆中并在长末端重复区域中。 CDNA克隆中的异质性进一步证明了在分化期间活化至少两个不同的IAP基因。分析核中的转录速率分析表明，PE样细胞中IAP基因的表达是转录调控的结果。这些观察结果表明，IAP转录的调节是自主调节的，而不是通过与发育调节细胞基因相邻的IAP序列的偶然集成。

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