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首页> 外文期刊>Molecular and Cellular Biology >Activated v-myc and v-ras oncogenes do not transform normal human lymphocytes.
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Activated v-myc and v-ras oncogenes do not transform normal human lymphocytes.

机译：活化的V-myc和V-ras oncogenes不会改变正常人淋巴细胞。

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Activated v-myc (pSV v-myc) and v-Ha-ras (GT10) oncogenes were introduced into normal human lymphocytes, NIH 3T3 fibroblasts, B-lymphoblastoid cells, and human epithelial cells, using a reconstituted Sendai virus envelope-mediated gene transfer technique. Efficient transfer of the plasmid in each cell type was demonstrable within 1.5 h of transfection by Southern blotting of extrachromosomal DNA extracts, which unexpectedly revealed that v-myc plasmid DNA was unstable in normal lymphocytes but not in the other cell types. The v-myc plasmid was stabilized when cotransfected into lymphocytes together with v-Ha-ras. The transfected v-Ha-ras plasmid was stable in all the cell types tested. v-myc plasmid expression was clearly detectable by 5 h in all cell types except human lymphocytes. Lymphocytes expressed v-myc when transfected together with v-Ha-ras. Transfected ras oncogene was efficiently expressed in all the cell types tested. Expression of the transfected genes increased at 24 and 48 h after transfection. Even though plasmid stability and expression were achieved in myc-ras-cotransfected lymphocytes, no effects on cellular DNA synthesis or immortalization were observed, in contrast to efficient transformation of NIH 3T3 fibroblasts by the same procedure. Our data suggest that efficient expression of transfected myc and ras oncogenes in normal quiescent human lymphocytes is not sufficient for the induction of cell growth and immortalization.
机译：使用重构的仙台病毒包膜介导的基因将活化的V-MYC（PSV V-MYC）和V-HA-RAS（GT10）孔生成为正常人淋巴细胞，NIH 3T3成纤维细胞，B淋巴细胞和人上皮细胞转移技术。在每种细胞类型中的高效转移在每种细胞类型的1.5小时内通过南部染色体DNA提取物的南部印迹转染，意外显示V-Myc质粒DNA在正常淋巴细胞中不稳定，但不在其他细胞类型中。当与V-HA-Ras一起分为淋巴细胞时，稳定V-MYC质粒。在测试的所有细胞类型中，转染的V-HA-RAS质粒稳定。在除了人淋巴细胞之外的所有细胞类型中，5小时明显可检测到V-Myc质粒表达。淋巴细胞在与V-HA-RA一起转染时表达V-MYC。在测试的所有细胞类型中有效地表达转染的Ras癌基因。转染后24和48小时的转染基因的表达增加。尽管在Myc-Ras-CoTransfected淋巴细胞中实现了质粒稳定性和表达，但对于通过相同程序的有效转化NIH 3T3成纤维细胞的有效转化，没有对细胞DNA合成或永生化的影响。我们的数据表明，正常静态人淋巴细胞中转染的Myc和Ras癌基因的有效表达是不足的，对于诱导细胞生长和永生化是不足的。

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