The 65,000-Mr DNA-binding and virion trans-inducing proteins of herpes simplex virus type 1.

H S Marsden; M E Campbell; L Haarr; M C Frame; D S Parris; M Murphy; R G Hope; M T Muller; C M Preston

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The 65,000-Mr DNA-binding and virion trans-inducing proteins of herpes simplex virus type 1.

机译：65,000-mR DNA结合和病毒肾上腺诱导蛋白单纯疱疹病毒1型。

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The possible identity of the herpes simplex virus type 1 (HSV-1) 65K (65,000-Mr) virion protein which stimulates transcription from immediate-early genes with the HSV-1 65K DNA-binding protein was investigated. The two proteins were found to be distinct by the three separate criteria of immunological reactivity, tryptic peptide fingerprinting, and mobility in two-dimensional gels. Using HSV-1/HSV-2 intertypic recombinants and a serotype-specific antiserum, we located the gene encoding the 65K DNA-binding protein between coordinates 0.574 and 0.682 on the HSV-1 genome. The protein is posttranslationally modified by phosphorylation. In crude extracts of HSV-1-infected cells the 65K trans-inducing protein did not detectably bind to double-stranded calf thymus DNA under the conditions of our assay.

机译：研究了单纯疱疹病毒类型1（HSV-1）65K（65,000-mR）病毒蛋白蛋白的可能身份，刺激来自HSV-1 65K DNA结合蛋白的立即早期基因的转录。发现两种蛋白质是不同的免疫反应性，胰蛋白酶肽指纹图谱和二维凝胶中的迁移率的三种单独标准。使用HSV-1 / HSV-2 Intertypic重组剂和血清型抗血清，我们位于在HSV-1基因组上编码65K DNA结合蛋白的基因。通过磷酸化进行后期改性蛋白质。在HSV-1感染的细胞的粗提取物中，在我们的测定条件下，65K型诱导蛋白在我们的测定条件下没有可检测到双链小牛胸腺DNA。

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《Journal of Virology》 |1987年第8期|共10页
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H S Marsden; M E Campbell; L Haarr; M C Frame; D S Parris; M Murphy; R G Hope; M T Muller; C M Preston;
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1. The 65,000-Mr DNA-binding and virion trans-inducing proteins of herpes simplex virus type 1. [J] . H S Marsden, M E Campbell, L Haarr, Journal of Virology . 1987,第8期

机译：65,000-mR DNA结合和病毒肾上腺诱导蛋白单纯疱疹病毒1型。
2. The 65,000-Mr DNA-binding and virion trans-inducing proteins of herpes simplex virus type 1. [J] . H S Marsden, M E Campbell, L Haarr, Journal of Virology . 1987,第8期

机译：65,000-mR DNA结合和病毒肾上腺诱导蛋白单纯疱疹病毒1型。
3. Characterization of the major mRNAs transcribed from the genes for glycoprotein B and DNA-binding protein ICP8 of herpes simplex virus type 1. [J] . L F Rafield, D M Knipe Journal of Virology . 1984,第3期

机译：从丙蛋白B和单纯疱疹病毒1型的糖蛋白B和DNA结合蛋白ICP8转录的主要MRNA的表征。
4. Characterization of VP26 Capsid Protein during Herpes Simplex Virus Type 1 Assembly [C] . Daniell L. Rowles, Todd M. Greco, Aaron E. Lin, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2011

机译：疱疹病毒型1组装疱疹病毒中VP26辣椒蛋白的表征
5. Beyond host shut-off: Identification of a novel role for the virion host shut-off (vhs) protein of herpes simplex virus type 1 (HSV-1) in immune evasion [D] . Cotter, Christopher 2011

机译：超越宿主关闭：鉴定单纯疱疹病毒1型（HSV-1）的病毒体宿主关闭（vhs）蛋白在免疫逃逸中的新作用
6. The 65000-Mr DNA-binding and virion trans-inducing proteins of herpes simplex virus type 1. [O] . H S Marsden, M E Campbell, L Haarr, 1987

机译：1型单纯疱疹病毒的65000-Mr DNA结合和病毒粒子转导蛋白。
7. Purification of the herpes simplex virus type 1 65-kilodalton DNA-binding protein: properties of the protein and evidence of its association with the virus-encoded DNA polymerase [O] . M L Gallo, D H Jackwood, M Murphy, 1988

机译：纯化单纯疱疹病毒1型65千杆菌DNA结合蛋白：蛋白质的性质和与病毒编码的DNA聚合酶的关系的证据

The 65,000-Mr DNA-binding and virion trans-inducing proteins of herpes simplex virus type 1.

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