• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Identification and isolation of the gene encoding the small subunit of ribonucleotide reductase from Saccharomyces cerevisiae: DNA damage-inducible gene required for mitotic viability.
【24h】

Identification and isolation of the gene encoding the small subunit of ribonucleotide reductase from Saccharomyces cerevisiae: DNA damage-inducible gene required for mitotic viability.

机译：鉴定和分离编码核糖核苷酸还原酶的小亚基的基因：毒性生存能力所需的DNA损伤诱导基因。

获取原文
       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Ribonucleotide reductase catalyzes the first step in the pathway for the production of deoxyribonucleotides needed for DNA synthesis. The gene encoding the small subunit of ribonucleotide reductase was isolated from a Saccharomyces cerevisiae genomic DNA expression library in lambda gt11 by a fortuitous cross-reaction with anti-RecA antibodies. The cross-reaction was due to an identity between the last four amino acids of each protein. The gene has been named RNR2 and is centromere linked on chromosome X. The nucleotide sequence was determined, and the deduced amino acid sequence, 399 amino acids, shows extensive homology with other eucaryotic ribonucleotide reductases. Transplason mutagenesis was used to disrupt the RNR2 gene. A novel assay using colony color sectoring was developed to demonstrate visually that RNR2 is essential for mitotic viability. RNR2 encodes a 1.5-kilobase mRNA whose levels increase 18-fold after treatment with the DNA-damaging agent 4-nitroquinoline 1-oxide. CDC8 was also found to be inducible by DNA damage, but POL1 and URA3 were not inducible by 4-nitroquinoline 1-oxide. The expression of these genes defines a new mode of regulation for enzymes involved in DNA biosynthesis and sharpens our picture of the events leading to DNA repair in eucaryotic cells.
机译：核糖核苷酸还原酶催化途径中的第一步，用于生产DNA合成所需的脱氧核苷酸。通过与抗RECA抗体的偶然交叉反应，在λGT11中的酿酒酵母酿酒酵母基因组DNA表达文库中分离编码核糖核苷酸还原酶小亚基的基因。交叉反应是由于每种蛋白质的最后四个氨基酸之间的身份。该基因已被命名为RNR2，并且是在染色体上连接的符号。测定核苷酸序列，并且推导的氨基酸序列399氨基酸显示出与其他基核糖核苷酸还原酶的广泛同源性。转膜诱变用于破坏RNR2基因。开发了使用菌落界的一种新型测定，以在视觉上证明RNR2对于有丝分裂生存力至关重要。 RNR2编码1.5千碱基mRNA，其水平在用DNA损伤剂4-​​硝基喹啉1-氧化物处理后增加18倍。发现CDC8通过DNA损伤诱导，但POL1和URA3不受4-硝基喹啉1-氧化物诱导。这些基因的表达定义了涉及DNA生物合成中涉及的酶的新调节模式，并锐化我们在核心细胞中导致DNA修复的事件的图片。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号