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Identification of a sulfate metabolite of PCB 11 in human serum

机译：人血清中PCB 11的硫酸盐代谢产物的鉴定

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摘要

Despite increasing evidence for a major role for sulfation in the metabolism of lower-chlorinated polychlorinated biphenyls in vitro and in vivo, and initial evidence for potential bioactivities of the resulting sulfate ester metabolites, the formation of PCB sulfates in PCB exposed human populations had not been explored. The primary goal of this study was to determine if PCB sulfates, and potentially other conjugated PCB derivatives, are relevant classes of PCB metabolites in the serum of humans with known exposures to PCBs. In order to detect and quantify dichlorinated PCB sulfates in serum samples of 46 PCB-exposed individuals from either rural or urban communities, we developed a high-resolution mass spectrometry-based protocol using 4-PCB 11 sulfate as a model compound. The method also allowed the preliminary analysis of these 46 human serum extracts for the presence of other metabolites, such as glucuronic acid conjugates and hydroxylated PCBs. Sulfate ester metabolites derived from dichlorinated PCBs were detectable and quantifiable in more than 20% of analyzed serum samples. Moreover, we were able to utilize this method to detect PCB glucuronides and hydroxylated PCBs, albeit at lower frequencies than PCB sulfates. Altogether, our results provide initial evidence for the presence of PCB sulfates in human serum. Considering the inability of previously employed analytical protocols for PCBs to extract these sulfate ester metabolites and the concentrations of these metabolites observed in our current study, our data support the hypothesis that total serum levels of PCB metabolites in exposed individuals may have been underestimated in the past. (C) 2016 Elsevier Ltd. All rights reserved.
机译：尽管有越来越多的证据表明硫酸盐在体外和体内在低级氯化多氯联苯的代谢中起主要作用，并且有初步证据表明所产生的硫酸酯代谢物具有潜在的生物活性，但尚未发现PCB硫酸盐在暴露于PCB的人群中的形成。探索。这项研究的主要目的是确定PCB硫酸盐和可能的其他共轭PCB衍生物是否是已知暴露于PCB的人类血清中相关类别的PCB代谢物。为了检测和量化来自农村或城市社区的46名PCB接触者的血清样本中的二氯PCB硫酸盐，我们开发了一种基于高分辨率质谱的方案，使用4-PCB 11硫酸盐作为模型化合物。该方法还可以对这46种人血清提取物进行初步分析，以确定是否存在其他代谢产物，例如葡萄糖醛酸结合物和羟基化PCB。来自二氯多氯联苯的硫酸酯代谢物在超过20％的分析血清样品中可检测和定量。此外，我们能够利用这种方法检测PCB中的葡糖醛酸苷和羟基化PCB，尽管其频率低于PCB硫酸盐。总之，我们的结果为人血清中PCB硫酸盐的存在提供了初步证据。考虑到以前无法使用的PCB分析方法无法提取这些硫酸酯代谢物以及在我们当前的研究中观察到的这些代谢物的浓度，我们的数据支持以下假设：过去暴露的个体中PCB代谢物的总血清水平可能被低估了。 （C）2016 Elsevier Ltd.保留所有权利。

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  • 来源
    《Environment international》 |2017年第1期|120-128|共9页
  • 作者

    Grimm Fabian A.; Lehmler Hans-Joachim; Koh Wen Xin; DeWall Jeanne; Teesch Lynn M.; Hornbuckle Keri C.; Thorne Peter S.; Robertson Larry W.; Duffel Michael W.;

  • 作者单位

    Univ Iowa, Interdisciplinary Grad Program Human Toxicol, Iowa City, IA USA|Univ Iowa, Coll Pharm, Dept Pharmaceut Sci & Expt Therapeut, 115 S Grand Ave,S325, Iowa City, IA 52246 USA;

    Univ Iowa, Interdisciplinary Grad Program Human Toxicol, Iowa City, IA USA|Univ Iowa, Coll Publ Hlth, Dept Occupat & Environm Hlth, Iowa City, IA USA;

    Univ Iowa, Interdisciplinary Grad Program Human Toxicol, Iowa City, IA USA|Univ Iowa, Coll Engn, Dept Civil & Environm Engn, Iowa City, IA 52242 USA;

    Univ Iowa, Coll Publ Hlth, Dept Occupat & Environm Hlth, Iowa City, IA USA;

    Univ Iowa, High Resolut Mass Spectrometry Facil, Iowa City, IA USA;

    Univ Iowa, Interdisciplinary Grad Program Human Toxicol, Iowa City, IA USA|Univ Iowa, Coll Engn, Dept Civil & Environm Engn, Iowa City, IA 52242 USA;

    Univ Iowa, Interdisciplinary Grad Program Human Toxicol, Iowa City, IA USA|Univ Iowa, Coll Publ Hlth, Dept Occupat & Environm Hlth, Iowa City, IA USA;

    Univ Iowa, Interdisciplinary Grad Program Human Toxicol, Iowa City, IA USA|Univ Iowa, Coll Publ Hlth, Dept Occupat & Environm Hlth, Iowa City, IA USA;

    Univ Iowa, Interdisciplinary Grad Program Human Toxicol, Iowa City, IA USA|Univ Iowa, Coll Pharm, Dept Pharmaceut Sci & Expt Therapeut, 115 S Grand Ave,S325, Iowa City, IA 52246 USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Polychlorinated biphenyls; PCB; Metabolism; Exposure assessment; Sulfation;

    机译：多氯联苯;多氯联苯;代谢;接触评估;硫酸盐;

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