...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental engineering and management journal >ENZYMATIC PROCESSING OF CHITINACEOUS WASTES FOR N-ACETYL-D-GLUCOSAMINE PRODUCTION: AN EXAMPLE OF GREEN AND EFFICIENT ENVIRONMENTAL MANAGEMENT
【24h】

ENZYMATIC PROCESSING OF CHITINACEOUS WASTES FOR N-ACETYL-D-GLUCOSAMINE PRODUCTION: AN EXAMPLE OF GREEN AND EFFICIENT ENVIRONMENTAL MANAGEMENT

机译：N-乙酰基-D-葡萄糖胺生产中几丁质废料的酶法处理：绿色高效的环境管理实例

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Chitin from chitinaceous prawn shell waste, fungal biomass of Aspergillus fumigatus and Penicillium waksmanii was used as natural substrate for partially purified chitinase of Trichoderma harzianum to produce N-acetyl-D-glucosamine (GlcNAc), a medically important amino sugar. The isolated chitins were characterized with FTTR and XRD for quality check. The enzymatic hydrolysis of isolated chitins was optimized at pH 5 and 37 ℃. For GlcNAc production from prawn waste, the standardized incubation duration was 4 days, while that for the fungal biomass was 3 days. The standardized enzyme concentration for biocatalytic based hydrolysis of isolated chitin was 4U/mL, irrespective of their source. The substrate amount was standardized at 17.5 mg/mL of reaction mixtures. The GlcNAc production was confirmed by TLC and HPLC and 80% conversion of isolated chitin from prawn wastes, 68% conversion of isolated chitin from Penicillium waksmanii biomass and 77 % conversion of isolated chitin from Aspergillus fumigatus biomass into GlcNAc were established by enzymatic hydrolysis.
机译：来自甲壳类虾壳废料的几丁质，烟曲霉和沃克斯曼青霉的真菌生物质被用作天然纯化哈茨木霉几丁质酶的天然底物，以生产N-乙酰基-D-葡萄糖胺（GlcNAc），一种医学上重要的氨基糖。使用FTTR和XRD对分离的几丁质进行表征，以进行质量检查。在5和37℃的pH下优化分离几丁质的酶解。从虾粪中生产GlcNAc的标准温育时间为4天，而真菌生物质的标准温育时间为3天。分离来源的几丁质基于生物催化水解的标准酶浓度为4U / mL，无论其来源如何。将底物量标准化为17.5 mg / mL反应混合物。通过TLC和HPLC确认了GlcNAc的产生，并且通过酶促水解建立了从虾虾中分离的几丁质的80％转化，从Waksmanii青霉生物质的分离的几丁质的68％转化以及从烟曲霉生物质的分离的几丁质向GlcNAc的77％的转化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号