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Pyrosequencing Analysis of Bench-Scale Nitrifying Biofilters Removing Trihalomethanes

机译:台式硝化生物滤池去除三卤甲烷的焦磷酸测序分析

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摘要

Biofilm communities in four bench-scale nitrifying biofilters degrading drinking water-regulated trihalomethanesnwere analyzed by next-generation high-throughput 454 pyrosequencing. On average, the bacterial communitiesnwere dominated by Nitrosomonas (69%), Nitrobacter (14%), and Chitinophagaceae (9%) with thendominant bacterial operational taxonomic unit (OTU) related to the ammonia-oxidizing bacteria (AOB) Nitrosomonasnoligotropha. Even though ammonia-oxidizing archaea (AOA) have been reported in drinking waternsystems, the current study generated no PCR product or a very low yield of detectable Archaea amplicons,nwhich may be attributed to biofilter operating conditions [e.g., 4mg N/L TOTNH3 (sum of ammonia-nitrogennand ammonium-nitrogen)] that promoted AOB compared with AOA growth. Spatially within a given biofiltern(influent to the effluent), OTU relative abundance significantly changed such that Nitrosomonas relative abundancendecreased and Chitinophagaceae and Nitrobacter relative abundance increased.
机译:通过下一代高通量454焦磷酸测序分析了四个台式规模硝化生物滤池中降解饮用水调节的三卤甲烷的生物膜群落。平均而言,细菌群落主要由亚硝化单胞菌(69%),硝化细菌(14%)和几丁香科(9%)以及与氨氧化细菌(AOB)亚硝化单胞菌寡核苷酸有关的主要细菌操作生物分类单位(OTU)主导。尽管饮用水系统中已经报道了氨氧化古细菌(AOA),但本研究未产生PCR产物或可检测的古细菌扩增子的产率很低,这可能归因于生物滤池的运行条件(例如4mg N / L TOTNH3( (氨氮和铵氮的总和)]促进了AOB的生长(与AOA的生长相比)。在给定的生物滤池(进水)中,OTU的相对丰度发生了显着变化,以致亚硝化单胞菌相对丰度降低,甲壳菌和硝化细菌的相对丰度增加。

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