• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental Engineering Science >Autonomous, Wireless In-Situ Sensor (AWISS) for Rapid Warning of Escherichia coli Outbreaks in Recreational and Source Waters
【24h】

Autonomous, Wireless In-Situ Sensor (AWISS) for Rapid Warning of Escherichia coli Outbreaks in Recreational and Source Waters

机译：自主的无线原位传感器（AWISS），用于快速预警娱乐水和水源水中大肠杆菌的暴发

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

An autonomous, wireless, in-situ sensor (AWISS) was demonstrated for the detection of Escherichia coli. Anprototype battery-powered optical AWISS, consisting of a miniature spectrophotometer, monitored the changesnin fluorescence intensity that occur when the E. coli–synthesized b-glucuronidase enzyme hydrolyzes the reagent’snglycosidic bond, releasing fluorophores into solution. Laboratory testing with the prototype sensornshowed the AWISS was capable of detecting low concentrations ( < 100 colony forming units [CFU]/100 mL) innless than eight hours. Higher concentrations ( > 5000 CFU/100 mL), indicative of a possible combined sewagendischarge, could be detected in under one hour. A seven-day demonstration utilized the prototype sensor tonsample and record the bacterial water quality of the St. Joseph River in South Bend, Indiana. Every six hours annew sample was drawn into a custom-designed stop-flow reaction chamber and injected with a soluble reagent.nA wireless transmitter sent intensity data to a remote monitoring station. The deployment produced 19 individualnsampling events, of which 15 were compared to culture methods approved by the U.S. EnvironmentalnProtection Agency (USEPA). Four samples were drawn in the middle of the night and not verified. Of the 15nverified samples, 13 correctly identified the presence or absence of an E. coli concentration defined as significantnby the USEPA and Indiana Department of Environmental Management (IDEM) thresholds of 125 CFU/100 mL.
机译：自主自主的无线原位传感器（AWISS）被证明可用于检测大肠杆菌。由原型电池供电的光学AWISS（由微型分光光度计组成）可监测大肠杆菌合成的b-葡糖醛酸苷酶水解酶的糖苷键并将荧光团释放到溶液中时荧光强度的变化。使用原型传感器的实验室测试表明，AWISS能够在不到8小时的时间内检测到低浓度（<100个菌落形成单位[CFU] / 100 mL）。一小时内可以检测到更高的浓度（> 5000 CFU / 100 mL），表明可能存在污水排放总量。为期七天的演示利用原型传感器tonsample并记录了印第安纳州南本德市圣约瑟夫河的细菌水质量。每六个小时，将新样品抽入定制设计的停止流反应室中，并注入可溶试剂。无线发射器将强度数据发送到远程监测站。部署产生了19个单独的采样事件，其中有15个与美国环境保护局（USEPA）批准的培养方法进行了比较。在深夜抽取了四个样本，未经验证。在15个经过验证的样本中，有13个正确地识别了是否存在由USEPA和印第安纳州环境管理部门（IDEM）定义为125 CFU / 100 mL阈值的大肠杆菌浓度。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号