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首页> 外文期刊>Environmental Pollution >Effects of eutrophication on vitellogenin gene expression in male fathead minnows (Pimephales promelas) exposed to 17 alpha-ethynylestradiol in field mesocosms
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Effects of eutrophication on vitellogenin gene expression in male fathead minnows (Pimephales promelas) exposed to 17 alpha-ethynylestradiol in field mesocosms

机译：富营养化对暴露于田间中毒的17α-乙炔雌二醇的雄性黑头呆fat（Pimephales promelas）卵黄蛋白原基因表达的影响

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摘要

This study evaluated the effect of aquatic secondary nutrient supply levels (nitrogen and phosphorus) on the subcellular response of adult male fathead minnows (Pimephales promelas) exposed to a single nominal concentration of 17 alpha-ethynytestradiol (EE2), a potent synthetic estrogen, under quasi-natural field conditions. Outdoor mesocosms were maintained under low, medium, and high nutrient supply conditions as categorized by total phosphorus (TP) level (nominal 0.012, 0.025, and 0.045 mg TP/L, respectively), and treated with EE2 with and without a carrier solvent. Using reverse transcription-polymerase chain reaction methods, vitellogenin gene (Vg) expression was determined in the fish collected at 0 h, 8 h, 24 h, 4 d, 7 d, and 14 d post-exposure. Induction of Vg was detected as early as 8 It post-exposure, with and without the carrier solvent, and persisted through Day 14. Results showed Vg to be significantly greater at low nutrient levels (p < 0.05), suggesting that EE2 bioavailability to the fish was likely greater under less-turbid water conditions. Published by Elsevier Ltd.
机译：这项研究评估了在单一标称浓度的有效合成雌激素下，暴露于单一有效浓度17α-乙炔睾丸醇（EE2）的水生次要营养物供应水平（氮和磷）对成年雄性黑头小min（Pimephales promelas）的亚细胞反应的影响。准自然场条件。根据总磷（TP）含量（分别标称值为0.012、0.025和0.045 mg TP / L）分类，将室外中观维持在低，中和高养分供应条件下，并在有和没有载体溶剂的情况下用EE2处理。使用逆转录-聚合酶链反应方法，在暴露后0 h，8 h，24 h，4 d，7 d和14 d收集的鱼中测定卵黄蛋白原基因（Vg）的表达。在有或没有载体溶剂的情况下，暴露后最早在8 It时就检测到Vg的诱导，并持续到第14天。结果显示，在低营养水平下Vg显着更高（p <0.05），表明EE2生物利用在不浑浊的水质条件下，鱼类可能更大。由Elsevier Ltd.发布

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