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首页> 外文期刊>Environmental Pollution >The role of miR-21 in nickel nanoparticle-induced MMP-2 and MMP-9 production in mouse primary monocytes: In vitro and in vivo studies
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The role of miR-21 in nickel nanoparticle-induced MMP-2 and MMP-9 production in mouse primary monocytes: In vitro and in vivo studies

机译：miR-21在小鼠初级单核细胞中镍纳米粒子诱导的MMP-2和MMP-9产生的作用：体外和体内研究

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摘要

Exposure to metal nanoparticles causes both pulmonary and systemic effects. Nanoparticles can enter the circulation and act directly or indirectly on blood cells, such as monocytes. Monocytes/macrophages are among the first cells to home to inflammatory sites and play a key role in the immune response. Here we investigated the effects of nickel nanoparticles (Nano-Ni), partially [O]-passivated Nano-Ni (Nano-Ni P), and carbon-coated Nano-Ni (Nano-Ni-C) on MMP-2 and MMP-9 production in mouse primary monocytes both in vitro and in vivo and explored the potential mechanisms involved. The doseand time-response studies showed that exposure of primary monocytes from wild-type (WT) mice to 30 mg/ mL of Nano-Ni for 24 h caused significant MMP-2 and MMP-9 production; therefore, these dose and time point were chosen for the following in vitro studies. Nano-Ni and Nano-Ni-P caused miR-21 upregulation, as well as MMP-2, MMP-9, TIMP-1 and TIMP-2 upregulation in monocytes from WT, but not miR-21 knock-out (KO), mice, indicating the important role of miR-21 in Nano-Ni-induced MMPs and TIMPs upregulation. However, Nano-Ni-C did not cause these effects, suggesting surface modification of Nano Ni, such as carbon coating, alleviates Nano-Ni-induced miR-21 and MMPs upregulation. These results were further confirmed by in vivo studies by intratracheal instillation of nickel nanoparticles into WT and miR-21 KO mice. Finally, our results demonstrated that exposure of primary monocytes from WT mice to Nano-Ni and Nano-Ni-P caused downregulation of RECK, a direct miR-21 target, suggesting the involvement of miR-21/RECK pathway in Nano-Ni-induced MMP-2 and MMP-9 production. (c) 2020 Elsevier Ltd. All rights reserved.
机译：暴露于金属纳米粒子会导致肺部和全身效应。纳米颗粒可以直接或间接地进入血液细胞，例如单核细胞。单核细胞/巨噬细胞是炎症部位的第一个细胞中，并在免疫应答中发挥关键作用。在这里，我们研究了镍纳米颗粒（纳米Ni），部分[O] - 普通纳米-NI（纳米-NIP）和碳涂覆的纳米Ni（NaNO-Ni-C）对MMP-2和MMP的影响-9在体外和体内的小鼠初级单核细胞中产生并探索所涉及的潜在机制。 Doseand时间响应研究表明，源自野生型（WT）小鼠的初级单核细胞暴露于30mg / ml纳米Ni 24小时，导致显着的MMP-2和MMP-9产生;因此，这些剂量和时间点被选择用于以下体外研究。纳米Ni和纳米Ni-P导致MiR-21上调，以及来自WT的单核细胞的MMP-2，MMP-9，TIMP-1和TIMP-2上调，但不是MIR-21敲除（KO）小鼠，表明miR-21在纳米Ni诱导的MMP和Timps上调中的重要作用。然而，纳米Ni-C没有引起这些效果，表明纳米Ni的表面改性，例如碳涂层，减轻纳米Ni诱导的miR-21和MMP上调。通过体内研究进一步证实了这些结果，通过腹腔内滴注到WT和miR-21ko小鼠中。最后，我们的结果表明，从WT小鼠的初级单核细胞暴露于纳米Ni和纳米Ni-P，导致Reck的直接MiR-21靶标的较高，表明MiR-21 / Reck途径在纳米 - 诱导MMP-2和MMP-9的生产。 （c）2020 elestvier有限公司保留所有权利。

著录项

  • 来源
    《Environmental Pollution》 |2020年第2期|115597.1-115597.13|共13页
  • 作者

    Mo Yiqun; Zhang Yue; Mo Luke; Wan Rong; Jiang Mizu; Zhang Qunwei;

  • 作者单位

    Univ Louisville Sch Publ Hlth & Informat Sci Dept Environm & Occupat Hlth Sci Louisville KY 40292 USA;

    Univ Louisville Sch Publ Hlth & Informat Sci Dept Environm & Occupat Hlth Sci Louisville KY 40292 USA;

    Univ Louisville Sch Publ Hlth & Informat Sci Dept Environm & Occupat Hlth Sci Louisville KY 40292 USA;

    Univ Louisville Sch Publ Hlth & Informat Sci Dept Environm & Occupat Hlth Sci Louisville KY 40292 USA;

    Univ Louisville Sch Publ Hlth & Informat Sci Dept Environm & Occupat Hlth Sci Louisville KY 40292 USA;

    Univ Louisville Sch Publ Hlth & Informat Sci Dept Environm & Occupat Hlth Sci Louisville KY 40292 USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Nickel nanoparticles (Nano-Ni); miR-21; Monocytes; MMP-2; MMP-9;

    机译：镍纳米粒子（纳米Ni）;miR-21;单核细胞;MMP-2;MMP-9;

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