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首页> 外文期刊>Environmental research >Electrospun poly(methyl methacrylate)/polyaniline fibres as a support for laccase immobilisation and use in dye decolourisation
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Electrospun poly(methyl methacrylate)/polyaniline fibres as a support for laccase immobilisation and use in dye decolourisation

机译：电纺聚（甲基丙烯酸甲酯）/聚苯胺纤维作为漆酶固定的载体，并在染料脱脱机中使用

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摘要

Novel electrospun poly(methyl methacrylate)/polyaniline electrospun fibres were produced, characterised, modified, and used as a support for laccase immobilisation by two methods: adsorption and covalent binding. Effective deposition of laccase by both methods was confirmed by FTIR and CLSM results. Nevertheless, the main objective of the study was to select the most favourable immobilisation conditions and prepare heterogeneous biocatalysts with the best possible catalytic properties. The highest relative activity of enzymes immobilised by adsorption and covalent binding were obtained after 1 h of immobilisation using laccase solution at a concentration of 1 mg/mL, at pH 5 and 25 °C. It was found that the immobilised enzymes, which were present in amounts of 110 mg/g and 185 mg/g for systems with adsorbed and covalently bonded laccase respectively, exhibited slightly lower substrate affinity, and in consequence also a lower maximum reaction rate, than the free enzyme. The stability of laccase improved significantly upon immobilisation: both heterogeneous biocatalysts retained over 80% relative activity even after 10 repeated catalytic cycles and 30 days of storage. The obtained systems were used for decolourisation of Remazol Brilliant Blue R dye from a model aqueous solution, resulting in removal efficiencies of 87% and 58% using adsorbed and covalently bonded laccase, respectively. The described approach to the removal of textile dye from model solution is significant for the sustainable and environmentally friendly decolourisation of various compounds from wastewater.
机译：制备新型电纺聚（甲基丙烯酸甲酯）/聚苯胺电纺丝纤维，其特征化，用两种方法用作漆酶固定的载体：吸附和共价结合。通过FTIR和CLSM结果证实了两种方法的有效沉积两种方法。尽管如此，该研究的主要目的是选择最有利的固定条件，并用最佳可能的催化性能制备异质生物催化剂。在浓度为1mg / ml的浓度为1mg / ml，在pH5和25℃下，在1小时后，获得通过吸附和共价结合而固定的酶的最高相对活性。发现分别存在于110mg / g的量为110mg / g和185mg / g的固定化酶，其具有吸附和共价键合漆酶的体系，表现出略低较低的底物亲和力，结果也是较低的最大反应速率，而不是较低的反应速率游离酶。在固定过程中，漆酶的稳定性显着改善：异均匀生物催化剂即使在10次重复的催化循环和30天内储存后也保留超过80％的相对活性。所得体系用于从模型水溶液中脱唑亮蓝R染料的脱色，从而使用吸附和共价键合的漆酶中去除87％和58％的去除效率。所描述的方法从模型溶液中除去纺织品染料的方法对于废水中各种化合物的可持续和环保的脱色是显着的。

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  • 来源
    《Environmental research》 |2020年第5期|109332.1-109332.10|共10页
  • 作者

    Katarzyna Jankowska; Jakub Zdarta; Adam Grzywaczyk; Ewa Kijenska-Gawronska; Andrzej Biadasz; Teofil Jesionowski;

  • 作者单位

    Institute of Chemical Technology and Engineering Faculty of Chemical Technology Poznan University of Technology Berdychowo 4 PL-60965 Poznan Poland;

    Institute of Chemical Technology and Engineering Faculty of Chemical Technology Poznan University of Technology Berdychowo 4 PL-60965 Poznan Poland;

    Institute of Chemical Technology and Engineering Faculty of Chemical Technology Poznan University of Technology Berdychowo 4 PL-60965 Poznan Poland;

    Faculty of Materials Science and Engineering Warsaw University of Technology Woioska 141 PL-02507 Warsaw Poland;

    Institute of Physics Faculty of Materials Engineering and Technical Physics Poznan University of Technology Piotrowo 3 PL-60965 Poznan Poland;

    Institute of Chemical Technology and Engineering Faculty of Chemical Technology Poznan University of Technology Berdychowo 4 PL-60965 Poznan Poland;

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  • 正文语种 eng
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  • 关键词

    Laccase; Electrospun materials; Enzyme immobilisation; Decolourisation of dyes;

    机译：漆包;电纺材料;酶固定;染料的脱盐;

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