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首页> 外文期刊>Environmental Science & Technology >Abundance of Chlorinated Solvent and 1,4-Dioxane Degrading Microorganisms at Five Chlorinated Solvent Contaminated Sites Determined via Shotgun Sequencing
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Abundance of Chlorinated Solvent and 1,4-Dioxane Degrading Microorganisms at Five Chlorinated Solvent Contaminated Sites Determined via Shotgun Sequencing

机译：通过Shot弹枪测序确定五个氯化溶剂污染位点的氯化溶剂和1,4-二恶烷降解微生物的丰度

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Shotgun sequencing was used for the quantification of taxonomic and functional biomarkers associated with chlorinated solvent bioremediation in 20 groundwater samples (five sites), following bioaugmentation with SDC-9. The analysis determined the abundance of (1) genera associated with chlorinated solvent degradation, (2) reductive dehalogenase (RDases) genes, (3) genes associated with 1,4-dioxane removal, (4) genes associated with aerobic chlorinated solvent degradation, and (5) D. mccartyi genes associated with hydrogen and corrinoid metabolism. The taxonomic analysis revealed numerous genera previously linked to chlorinated solvent degradation, including Dehalococcoides, Desulfitobacterium, and Dehalogenimonas. The functional gene analysis indicated vcrA and tceA from D. mccartyi were the RDases with the highest relative abundance. Reads aligning with both aerobic and anaerobic biomarkers were observed across all sites. Aerobic solvent degradation genes, etnC or etnE, were detected in at least one sample from each site, as were pmoA and mmoX. The most abundant 1,4-dioxane biomarker detected was Methylosinus trichosporium OB3b mmoX. Reads aligning to thmA or Pseudonocardia were not found. The work illustrates the importance of shotgun sequencing to provide a more complete picture of the functional abilities of microbial communities. The approach is advantageous over current methods because an unlimited number of functional genes can be quantified.
机译：在使用SDC-9进行生物增强后，使用Shotgun测序来定量与20个地下水样品（五个位置）中的氯化溶剂生物修复有关的生物分类和功能生物标记。分析确定了（1）与氯化溶剂降解有关的属，（2）还原脱卤素酶（RDases）基因，（3）与1,4-二恶烷去除有关的基因，（4）与有氧氯化溶剂降解有关的基因， （5）与氢和类雌激素代谢有关的麦卡迪氏菌基因。分类学分析揭示了许多以前与氯化溶剂降解有关的属，包括脱卤球菌，脱硫杆菌和脱卤单胞菌。功能基因分析表明，来自麦卡氏菌的vcrA和tceA是相对丰度最高的RDase。在所有位点均观察到与需氧和厌氧生物标志物一致的读数。在每个位置的至少一个样品中检测到好氧溶剂降解基因etnC或etnE，pmoA和mmoX也是如此。检测到的最丰富的1,4-二恶烷生物标记物是毛果甲烷菌OB3b mmoX。找不到与thmA或假性心动过速一致的读物。这项工作说明了gun弹枪测序的重要性，以提供更完整的微生物群落功能能力的图片。该方法优于当前方法，因为可以定量无限数量的功能基因。

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