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首页> 外文期刊>Environmental Science & Technology >Down-Regulation of hspb9 and hspb11 Contributes to Wavy Notochord in Zebrafish Embryos Following Exposure to Polychlorinated Diphenylsulfides
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Down-Regulation of hspb9 and hspb11 Contributes to Wavy Notochord in Zebrafish Embryos Following Exposure to Polychlorinated Diphenylsulfides

机译：暴露于多氯二苯硫醚后，hspb9和hspb11的下调导致斑马鱼胚胎中的波浪脊索

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摘要

It is hypothesized that key genes, other than ahr2, are present and associated with the development of a unique type of notochord malformation known as wavy notochord in early life stages of zebrafish following exposure to polychlorinated diphenylsulfides (PCDPSs). To investigate the potential mechanism(s), time-dependent developmental morphologies of zebrafish embryos following exposure to 2500 nM 2,4,4',5-tetra-CDPS, 2,2',4-tri-CDPS or 4,4'-di-CDPS were observed to determine the developmental time point when notochord twists began to occur (i.e., 21 h-postfertilization (hpf)). Simultaneously, morphometric measurements suggested that PCDPS exposure did not affect notochord growth at 21 or 120 hpf; however, elongation of the body axis was significantly inhibited at 120 hpf. Transcriptome analysis revealed that the retardation of body growth was potentially related with dysregulation of transcripts predominantly associated with the insulin-associated Irs-Akt-FoxO cascade. Moreover, knockdown and gain-of-function experiments in vivo on codifferentially expressed genes demonstrated that reduced expression of hspb9 and hspb11 contributed to the occurrence of wavy notochord. The results of this study strongly support the hypothesis that the notochord kinks and twists are triggered by the down-regulation of hspb9 and hspb11, and intensified by body growth retardation along with normal notochord length in PCDPS-exposed zebrafish embryos.
机译：假设存在ahr2以外的关键基因，并且这些基因与斑马鱼生命早期暴露于多氯二苯硫醚（PCDPSs）之后的独特类型的脊索畸形（称为波浪脊索）的发展有关。为了研究斑马鱼胚胎在暴露于2500 nM 2,4,4'，5-tetra-CDPS，2,2'，4-tri-CDPS或4,4'之后的潜在机制，时间依赖性的发育形态观察到-di-CDPS来确定脊索扭转开始发生的发育时间点（即21 h后受精（hpf））。同时，形态计量学测量表明，PCDPS暴露在21或120 hpf时不影响脊索生长。然而，在120hpf时，体轴的伸长被显着抑制。转录组分析显示，机体生长的延迟可能与主要与胰岛素相关的Irs-Akt-FoxO级联相关的转录本失调有关。此外，在体内以共差异表达的基因进行的基因敲减和功能获得实验表明，hspb9和hspb11的表达降低导致波浪脊索的发生。这项研究的结果有力地支持了这样的假说，即脊索扭结和扭曲是由hspb9和hspb11的下调触发的，并且由于PCDPS暴露的斑马鱼胚胎中的体线发育缓慢以及正常的脊索长度而加剧了这种现象。

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  • 来源
    《Environmental Science & Technology》 |2018年第21期|12829-12840|共12页
  • 作者

    Zhang Rui; Wang Xiaoxiang; Zhang Xuesheng; Zhang Junjiang; Zhang Xiaowei; Shi Xiao; Crump Doug; Letcher Robert J.; Giesy John P.; Liu Chunsheng;

  • 作者单位

    Anhui Univ, Sch Resources & Environm Engn, Hefei 230601, Anhui, Peoples R China;

    Assoc Chinese Chemists & Chem Engineers Germany, D-67117 Limburgerhof, Germany;

    Southern Med Univ, Dept Obstet & Gynaecol, Nanfang Hosp, Ctr Reprod Med, Guangzhou 510515, Guangdong, Peoples R China;

    Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Hubei, Peoples R China;

    Univ Saskatchewan, Dept Vet Biomed Sci, Saskatoon, SK S7N 5B3, Canada;

    Carleton Univ, Ecotoxicol & Wildlife Hlth Div, Environm & Climate Change Canada, Natl Wildlife Res Ctr, 1125 Colonel By Dr, Ottawa, ON K1A 0H3, Canada;

    Univ Jinan, Sch Resources & Environm, Jinan 250022, Shandong, Peoples R China;

    Univ Saskatchewan, Toxicol Ctr, Saskatoon, SK S7N 5B3, Canada;

    Nanjing Univ, Sch Environm, State Key Lab Pollut Control & Resources Reuse, Nanjing 210023, Jiangsu, Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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