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Oxygen Half-Saturation Constants for Pharmaceuticals in Activated Sludge and Microbial Community Activity under Varied Oxygen Levels

机译:变动氧气水平下活性污泥中药物的氧气半饱和常数和微生物群落活性

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摘要

Aeration accounts for the largest energy demand in conventional activated sludge wastewater treatment. Emerging aeration control strategies for energy conservation have significantly reduced operational bulk liquid dissolved oxygen (DO) from above 2 mg/L to at or below 0.5 mg/L. As we move toward low DO treatment processes, there is a need to understand how low DO impacts the kinetics of micropollutant biotransformation. The objective of this study was to characterize the impact of DO concentration on pharmaceutical biotransformation rates via two approaches: (1) Determine oxygen half saturation constants that describe the community-wide impact of DO on biotransformation rates. (2) Evaluate shifts in the microbial community 16S rRNA pool due to DO concentration. Batch experiments were performed at several DO concentrations using biomass from a full-scale wastewater treatment plant. Results reveal that substantial reductions in bulk liquid DO concentrations to 0.5 mg-O-2/L are possible without compromising pharmaceutical biotransformation rates. Sequencing of cDNA generated from community rRNA revealed that diverse, low abundance community members may play important roles in pharmaceutical transformation. The results of this work advance our ability to predict and model the impact of DO on pharmaceutical biotransformations during wastewater treatment and identify taxonomic groups associated with those biotransformations.
机译:曝气是常规活性污泥废水处理中最大的能源需求。新兴的节能控制策略已将可操作的散装液体溶解氧(DO)从2 mg / L以上大大降低至0.5 mg / L或以下。随着我们朝着低溶解氧处理工艺发展,有必要了解低溶解氧如何影响微污染物生物转化的动力学。这项研究的目的是通过两种方法来表征DO浓度对药物生物转化率的影响:(1)确定氧半饱和常数,以描述DO对生物转化率的整个社区影响。 (2)评估由于DO浓度引起的微生物群落16S rRNA库的变化。使用来自大型废水处理厂的生物质,在几种DO浓度下进行了批量实验。结果表明,在不损害药物生物转化率的情况下,可以将大量液体DO浓度大幅降低至0.5 mg-O-2 / L。从社区rRNA生成的cDNA的测序表明,多样的,低丰度的社区成员可能在药物转化中起重要作用。这项工作的结果提高了我们预测和模拟溶解氧对废水处理过程中药物生物转化的影响并确定与那些生物转化相关的分类学类别的能力。

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  • 来源
    《Environmental Science & Technology》 |2019年第4期|1918-1927|共10页
  • 作者单位

    Univ Michigan, Dept Civil & Environm Engn, Ann Arbor, MI 48104 USA|Rice Univ, Dept Civil & Environm Engn, Houston, TX 77005 USA;

    Univ Michigan, Dept Civil & Environm Engn, Ann Arbor, MI 48104 USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
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