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首页> 外文期刊>Environmental Science & Technology >Adaptive Evolution of Escherichia coli to Gprofloxacin in Controlled Stress Environments: Contrasting Patterns of Resistance in Spatially Varying versus Uniformly Mixed Concentration Conditions
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Adaptive Evolution of Escherichia coli to Gprofloxacin in Controlled Stress Environments: Contrasting Patterns of Resistance in Spatially Varying versus Uniformly Mixed Concentration Conditions

机译：在受控压力环境下大肠杆菌对丙氧氟沙星的适应性进化：在空间变化和均匀混合浓度条件下的抗性对比模式

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摘要

A microfluidic gradient chamber (MGC) and a homogeneous batch culturing system were used to evaluate whether spatial concentration gradients of the antibiotic ciprofloxacin allow development of greater antibiotic resistance in Escherichia coli strain 307 (E. coli 307) compared to exclusively temporal concentration gradients, as indicated in an earlier study. A linear spatial gradient of ciprofloxacin and Luria-Bertani broth (LB) medium was established and maintained by diffusion over 5 days across a well array in the MGC, with relative concentrations along the gradient of 1.7-7.7X the original minimum inhibitory concentration (MICoriginal). The E. coli biomass increased in wells with lower ciprofloxacin concentrations, and only a low level of resistance to ciprofloxacin was detected in the recovered cells (similar to 2x MICoriginal). Homogeneous batch culture experiments were performed with the same temporal exposure history to ciprofloxacin concentration, the same and higher initial cell densities, and the same and higher nutrient (i.e., LB) concentrations as in the MGC. In all batch experiments, E. coli 307 developed higher ciprofloxacin resistance after exposure, ranging from 4 to 24X MICoriginal in all replicates. Hence, these results suggest that the presence of spatial gradients appears to reduce the driving force for E. coli 307 adaptation to ciprofloxacin, which suggests that results from batch experiments may over predict the development of antibiotic resistance in natural environments.
机译：与仅按时间浓度梯度相比，微流控梯度室（MGC）和均质分批培养系统用于评估环丙沙星抗生素的空间浓度梯度是否允许在大肠杆菌307菌株（大肠杆菌307）中产生更大的抗生素抗性。在较早的研究中指出。建立环丙沙星和Luria-Bertani肉汤（LB）培养基的线性空间梯度，并通过在MGC的孔阵列中扩散5天来保持，相对浓度沿原始最小抑制浓度的1.7-7.7X梯度变化（MICoriginal ）。环丙沙星浓度较低的孔中大肠杆菌生物量增加，并且在回收的细胞中仅检测到对环丙沙星的低水平抗性（类似于2x MICoriginal）。进行了均质的分批培养实验，其环丙沙星浓度的时间暴露历史相同，初始细胞密度相同且较高，初始养分（即LB）浓度较高且与MGC中相同。在所有批次实验中，大肠杆菌307暴露后产生的环丙沙星耐药性更高，所有重复试验的药敏度为4至24倍MICoriginal。因此，这些结果表明，空间梯度的存在似乎降低了大肠杆菌307对环丙沙星的适应性驱动力，这表明分批实验的结果可能会过度预测自然环境中抗生素耐药性的发展。

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  • 来源
    《Environmental Science & Technology》 |2019年第14期|7996-8005|共10页
  • 作者

    Deng Jinzi; Zhou Lang; Sanford Robert A.; Shechtman Lauren A.; Dong Yiran; Alcalde Reinaldo E.; Sivaguru Mayandi; Fried Glenn A.; Werth Charles J.; Fouke Bruce W.;

  • 作者单位

    Univ Illinois, Carl R Woese Inst Genom Biol, Urbana, IL 61801 USA;

    Univ Texas Austin, Dept Civil Architectural & Environm Engn, Austin, TX 78705 USA;

    Univ Illinois, Dept Geol, Urbana, IL 61801 USA;

    Univ Illinois, Dept Chem, Urbana, IL 61801 USA|Univ Illinois, Dept Integrat Biol, Urbana, IL 61801 USA;

    Univ Illinois, Carl R Woese Inst Genom Biol, Urbana, IL 61801 USA|China Univ Geosci Wuhan, Sch Environm Studies, Wuhan 430074, Hubei, Peoples R China;

    Univ Texas Austin, Dept Civil Architectural & Environm Engn, Austin, TX 78705 USA;

    Univ Illinois, Carl R Woese Inst Genom Biol, Urbana, IL 61801 USA;

    Univ Illinois, Carl R Woese Inst Genom Biol, Urbana, IL 61801 USA;

    Univ Texas Austin, Dept Civil Architectural & Environm Engn, Austin, TX 78705 USA;

    Univ Illinois, Carl R Woese Inst Genom Biol, Urbana, IL 61801 USA|Univ Illinois, Dept Geol, Urbana, IL 61801 USA|Univ Illinois, Dept Microbiol, Urbana, IL 61801 USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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