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首页> 外文期刊>Environmental Science & Technology >Exposure to Dimethyl Selenide (DMSe)-Derived Secondary Organic Aerosol Alters Transcriptomic Profiles in Human Airway Epithelial Cells
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Exposure to Dimethyl Selenide (DMSe)-Derived Secondary Organic Aerosol Alters Transcriptomic Profiles in Human Airway Epithelial Cells

机译：暴露于二甲基硒化物（DMSe）衍生的次级有机气溶胶会改变人类气道上皮细胞的转录组谱。

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摘要

Dimethyl selenide (DMSe) is one of the major volatile organoselenium compounds released from aquatic and terrestrial environments through microbial transformation and plant metabolism. The detailed processes of DMSe leading to secondary organic aerosol (SOA) formation and the pulmonary health effects induced by inhalation of DMSe-derived SOA remain largely unknown. In this study, we characterized the chemical composition and formation yields of SOA produced from the oxidation of DMSe with OH radicals and O-3 in controlled chamber experiments. Further, we profiled the transcriptome-wide gene expression changes in human airway epithelial cells (BEAS-2B) after exposure to DMSe-derived SOA. Our analyses indicated a significantly higher SOA yield resulting from the OH-initiated oxidation of DMSe. The oxidative potential of DMSe-derived SOA, as measured by the dithiothreitol (DTT) assay, suggested the presence of oxidizing moieties in DMSe-derived SOA at levels higher than typical ambient aerosols. Utilizing RNA sequencing (RNA-Seq) techniques, gene expression profiling followed by pathway enrichment analysis revealed several major biological pathways perturbed by DMSe-derived SOA, including elevated genotoxicity, DNA damage, and p53-mediated stress responses, as well as downregulated cholesterol biosynthesis, glycolysis, and interleukin IL-4/IL-13 signaling. This study highlights the significance of DMSe-derived SOA as a stressor in human airway epithelial cells.
机译：硒化二甲酯（DMSe）是通过微生物转化和植物代谢从水生和陆地环境释放的主要挥发性有机硒化合物之一。 DMSe导致继发性有机气溶胶（SOA）形成的详细过程以及由DMSe衍生的SOA吸入引起的肺部健康影响仍然未知。在这项研究中，我们表征了在受控室实验中由OH自由基和O-3氧化DMSe所产生的SOA的化学组成和形成产率。此外，我们分析了暴露于DMSe的SOA后人气道上皮细胞（BEAS-2B）中转录组全基因表达的变化。我们的分析表明，由OH引发的DMSe氧化可显着提高SOA产量。通过二硫苏糖醇（DTT）分析测定，DMSe衍生的SOA的氧化电位表明，DMSe衍生的SOA中存在的氧化部分的含量高于典型的环境气溶胶水平。利用RNA测序（RNA-Seq）技术，基因表达谱分析以及随后的途径富集分析揭示了DMSe衍生的SOA干扰的几种主要生物学途径，包括遗传毒性升高，DNA损伤和p53介导的应激反应，以及胆固醇生物合成的下调。 ，糖酵解和白介素IL-4 / IL-13信号传导。这项研究强调了DMSe衍生的SOA在人气道上皮细胞中作为应激源的重要性。

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  • 来源
    《Environmental Science & Technology》 |2019年第24期|14660-14669|共10页
  • 作者

    Ahmed C. M. Sabbir; Cui Yumeng; Frie Alexander L.; Burr Abigail; Kamath Rohan; Chen Jin Y.; Rahman Arafat; Nordgren Tara M.; Lin Ying-Hsuan; Bahreini Roya;

  • 作者单位

    Univ Calif Riverside Environm Toxicol Grad Program Riverside CA 92521 USA;

    Univ Calif Riverside Dept Environm Sci Riverside CA 92521 USA;

    Univ Calif Riverside Div Biomed Sci Riverside CA 92521 USA;

    Univ Calif Riverside Genet Genom & Bioinformat Riverside CA 92521 USA;

    Univ Calif Riverside Environm Toxicol Grad Program Riverside CA 92521 USA|Univ Calif Riverside Div Biomed Sci Riverside CA 92521 USA;

    Univ Calif Riverside Environm Toxicol Grad Program Riverside CA 92521 USA|Univ Calif Riverside Dept Environm Sci Riverside CA 92521 USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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