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首页> 外文期刊>Environmental Science & Technology >Gene Expression Profiling in Human Lung Cells Exposed to Isoprene-Derived Secondary Organic Aerosol
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Gene Expression Profiling in Human Lung Cells Exposed to Isoprene-Derived Secondary Organic Aerosol

机译：异戊二烯衍生的次级有机气溶胶暴露在人肺细胞中的基因表达谱

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摘要

Secondary organic aerosol (SOA) derived from the photochemical oxidation of isoprene contributes a substantial mass fraction to atmospheric fine particulate matter (PM_(2.5)). The formation of isoprene SOA is influenced largely by anthropogenic emissions through multiphase chemistry of its multigenerational oxidation products. Considering the abundance of isoprene SOA in the troposphere, understanding mechanisms of adverse health effects through inhalation exposure is critical to mitigating its potential impact on public health. In this study, we assessed the effects of isoprene SOA on gene expression in human airway epithelial cells (BEAS-2B) through an air-liquid interface exposure. Gene expression profiling of 84 oxidative stress and 249 inflammation-associated human genes was performed. Our results show that the expression levels of 29 genes were significantly altered upon isoprene SOA exposure under noncytotoxic conditions (p < 0.05), with the majority (22/29) of genes passing a false discovery rate threshold of 0.3. The most significantly affected genes belong to the nuclear factor (erythroid-derived 2)-like 2 (Nrf2) transcription factor network. The Nrf2 function is confirmed through a reporter cell line. Together with detailed characterization of SOA constituents, this study reveals the impact of isoprene SOA exposure on lung responses and highlights the importance of further understanding its potential health outcomes.
机译：源自异戊二烯的光化学氧化的二次有机气溶胶（SOA）对大气中的细颗粒物（PM_（2.5））的贡献很大。异戊二烯SOA的形成在很大程度上受其多代氧化产物的多相化学作用的人为排放影响。考虑到对流层中异戊二烯SOA的丰富性，了解通过吸入暴露对健康造成不利影响的机制对于减轻其对公共健康的潜在影响至关重要。在这项研究中，我们评估了异戊二烯SOA通过气液界面暴露对人气道上皮细胞（BEAS-2B）基因表达的影响。进行了84个氧化应激和249个与炎症相关的人类基因的基因表达谱分析。我们的研究结果表明，在无细胞毒性条件下，异戊二烯SOA暴露后29个基因的表达水平发生了显着变化（p <0.05），其中大多数（22/29）基因通过了0.3的错误发现率阈值。受影响最严重的基因属于核因子（类胡萝卜素衍生的2）样2（Nrf2）转录因子网络。 Nrf2功能是通过报告细胞系确认的。结合SOA成分的详细表征，该研究揭示了异戊二烯SOA暴露对肺部反应的影响，并强调了进一步了解其潜在健康结果的重要性。

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  • 来源
    《Environmental Science & Technology》 |2017年第14期|8166-8175|共10页
  • 作者

    Lin Yinghsuan; Arashiro Maiko; Clapp Phillip W.; Cui Tianqu; Sexton Kenneth G.; Vizuete William; Gold Avram; Jaspers Ilona; Fry Rebecca C.; Surratt Jason D.;

  • 作者单位

    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, NC, United States,Department of Environmental Sciences, Environmental Toxicology Graduate Program, University of California, Riverside, CA, United States;

    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, NC, United States,Department of Environmental Studies, Dickinson College, Carlisle, Philadelphia, PA, United States;

    Center for Environmental Medicine, Asthma and Lung Biology, School of Medicine, University of North Carolina, Chapel Hill, NC, United States;

    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, NC, United States;

    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, NC, United States;

    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, NC, United States;

    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, NC, United States;

    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, NC, United States,Center for Environmental Medicine, Asthma and Lung Biology, School of Medicine, University of North Carolina, Chapel Hill, NC, United States,Department of Pediatrics, School of Medicine, University of North Carolina, Chapel Hill, NC, United States;

    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, NC, United States;

    Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina, Chapel Hill, NC, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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