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首页> 外文期刊>Environmental Science & Technology >Evaluation of Microbiome-Host Relationships in the Zebrafish Gastrointestinal System Reveals Adaptive Immunity Is a Target of Bis(2-ethylhexyl) Phthalate (DEHP) Exposure
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Evaluation of Microbiome-Host Relationships in the Zebrafish Gastrointestinal System Reveals Adaptive Immunity Is a Target of Bis(2-ethylhexyl) Phthalate (DEHP) Exposure

机译：斑马鱼胃肠系统中微生物组-宿主关系的评估表明适应性免疫是邻苯二甲酸二（2-乙基己基）酯（DEHP）暴露的目标

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摘要

To improve physical characteristics of plastics such as flexibility and durability, producers enrich materials with phthalates such as di-2-(ethylhexyl) phthalate (DEHP). DEHP is a high production volume chemical associated with metabolic and immune disruption in animals and humans. To reveal mechanisms implicated in phthalate-related disruption in the gastrointestinal system, male and female zebrafish were fed DEHP (3 ppm) daily for two months. At the transcriptome level, DEHP significantly upregulated gene networks in the intestine associated with helper T cells' (Th1, Th2, and Th17) specific pathways. The activation of gene networks associated with adaptive immunity was linked to the suppression of networks for tight junction, gap junctional intercellular communication, and transmembrane transporters, all of which are precursors for impaired gut integrity and performance. On a class level, DEHP exposure increased Bacteroidia and Gammaproteobacteria and decreased Verrucomicrobiae in both the male and female gastrointestinal system. Further, in males there was a relative increase in Fusobaderiia and Betaproteobacteria and a relative decrease in Saccharibacteria. Predictive algorithms revealed that the functional shift in the microbiome community, and the metabolites they produce, act to modulate intestinal adaptive immunity. This finding suggests that the gut microbiota may contribute to the adverse effects of DEHP on the host by altering metabolites sensed by both intestinal and immune Th cells. Our results suggest that the microbiome-gut-immune axis can be modified by DEHP and emphasize the value of multiomics approaches to study microbiome-host interactions following chemical perturbations.
机译：为了改善塑料的物理特性（例如柔韧性和耐用性），生产商使用邻苯二甲酸盐（例如邻苯二甲酸二-2-（乙基己基）酯（DEHP））来丰富材料。 DEHP是与动物和人类的代谢和免疫破坏相关的高产量化学品。为了揭示胃肠道系统中邻苯二甲酸酯相关破坏的机制，每天给雄性和雌性斑马鱼喂食DEHP（3 ppm），持续两个月。在转录组水平上，DEHP显着上调了与辅助T细胞（Th1，Th2和Th17）特定途径相关的肠道基因网络。与适应性免疫相关的基因网络的激活与紧密连接，间隙连接细胞间通讯和跨膜转运蛋白网络的抑制有关，所有这些都是肠完整性和功能受损的先兆。在班级水平上，在男性和女性胃肠道系统中，DEHP暴露会增加细菌和γ-丙酸杆菌的数量，并降低Verrucomicrobiae。此外，在男性中，梭菌和贝氏变形杆菌相对增加，而糖细菌则相对减少。预测算法表明，微生物组社区的功能转变及其产生的代谢产物可调节肠道适应性免疫。这一发现表明，肠道菌群可能会通过改变肠道和免疫Th细胞所感知的代谢产物而对DEHP对宿主的不利影响做出贡献。我们的研究结果表明，DEHP可以修饰微生物组肠道免疫轴，并强调采用多组学方法研究化学扰动后微生物组与宿主之间的相互作用的价值。

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  • 来源
    《Environmental Science & Technology》 |2020年第9期|5719-5728|共10页
  • 作者

    Ondrej Adamovsky; Amanda N. Buerger; Hana Vespalcova; Shahadur R. Sohag; Amy T. Hanlon; Pamela E. Ginn; Serena L. Craft; Stanislav Smatana; Eva Rudinska; Maria Persico; Joseph H. Bisesi Jr.; Christopher J. Martyniuk;

  • 作者单位

    Research Centre for Toxic Compounds in the Environment (RECETOX) Masaryk University Brno Czech Republic Department of Physiological Sciences and Center for Environmental and Human Toxicology UF Genetics Institute College of Veterinary Medicine University of Florida Gainesville Florida 32611 United States;

    Department of Environmental and Global Health and Center for Environmental and Human Toxicology College of Public Health and Health Professions University of Florida Gainesville Florida United States;

    Research Centre for Toxic Compounds in the Environment (RECETOX) Masaryk University Brno Czech Republic;

    Department of Physiological Sciences and Center for Environmental and Human Toxicology UF Genetics Institute College of Veterinary Medicine University of Florida Gainesville Florida 32611 United States;

    Department of Comparative Diagnostic and Population Medicine College of Veterinary Medicine University of Florida Gainesville Florida United States;

    Research Centre for Toxic Compounds in the Environment (RECETOX) Masaryk University Brno Czech Republic Brno University of Technology Faculty of Information Technology IT4Innovations Centre of Excellence 61266 Brno Czech Republic;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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