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首页> 外文期刊>Environmental Science & Technology >Development and Application of a Novel Bioassay System for Dioxin Determination and Aryl Hydrocarbon Receptor Activation Evaluation in Ambient-Air Samples
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Development and Application of a Novel Bioassay System for Dioxin Determination and Aryl Hydrocarbon Receptor Activation Evaluation in Ambient-Air Samples

机译：用于环境空气样品中二恶英测定和芳烃受体活化评估的新型生物测定系统的开发和应用

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摘要

Airborne persistent toxic substances are associated with health impacts resulting from air pollution, for example, dioxins, dioxin-like polychlorinated biphenyls, and certain polycyclic aromatic hydrocarbons (PAHs), which activate aryl hydrocarbon receptors (AhR) and thereby produce adverse outcomes. Thus, a bioassay for evaluating AhR activation is required for risk assessment of ambient-air samples, and for this purpose, we developed a new and sensitive recombinant mouse hepatoma cell line, CBG2.8D, in which a novel luciferase-reporter plasmid containing two copies of a newly designed dioxin-responsive domain and a minimal promoter derived from a native gene were integrated. The minimal detection limit for 2,3,7,8-tetrachlorodibenzo- p -dioxin with this assay system was 0.1 pM. We used CBG2.8D to determine dioxin levels in 45 ambient-air samples collected in Beijing. The measured bioanalytical equivalent (BEQ) values were closely correlated with the toxic equivalent values obtained from chemical analysis. In haze ambient-air samples, the total activation of aryl hydrocarbon receptors (TAA) was considerably higher than the BEQ of dioxin-rich fractions, according to the results of the cell-based bioassay. Notably, the haze samples contained abundant amounts of PAHs, whose relative toxicity equivalent was correlated with the TAA; this finding suggests that PAHs critically contribute to the AhR-related biological impacts of haze ambient-air samples.
机译：空气中的持久性有毒物质与空气污染相关的健康影响，例如二恶英，类二恶英的多氯联苯和某些多环芳烃（PAH），它们会激活芳基烃受体（AhR），从而产生不良后果。因此，对于环境空气样品的风险评估，需要一种用于评估AhR激活的生物测定法，为此，我们开发了一种新型且敏感的重组小鼠肝癌细胞系CBG2.8D，其中一种新型荧光素酶报告质粒包含两个整合了一个新设计的二恶英响应域的拷贝和一个源自天然基因的最小启动子。用该测定系统对2,3,7,8-四氯二苯并-对二恶英的最低检出限为0.1 pM。我们使用CBG2.8D测定了北京收集的45个环境空气样品中的二恶英水平。测得的生物分析当量（BEQ）值与化学分析获得的毒性当量值密切相关。根据基于细胞的生物测定结果，在雾霾环境空气样品中，芳烃受体（TAA）的总活化度明显高于富二恶英级分的BEQ。值得注意的是，雾度样品中含有大量的PAH，其相对毒性当量与TAA相关。这一发现表明，多环芳烃对霾环境空气样品的与AhR相关的生物学影响至关重要。

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  • 来源
    《Environmental Science & Technology》 |2018年第5期|2926-2933|共8页
  • 作者

    Songyan Zhang; Shuaizhang Li; Zhiguang Zhou; Hualing Fu; Li Xu; Heidi Qunhui Xie; Bin Zhao;

  • 作者单位

    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China,University of Chinese Academy of Sciences, Beijing 100049, China;

    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China,University of Chinese Academy of Sciences, Beijing 100049, China;

    State Environmental Protection Key Laboratory of Dioxin Pollution Control, National Research Center for Environmental Analysis and Measurement, Beijing 100029, China;

    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China,University of Chinese Academy of Sciences, Beijing 100049, China;

    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China,University of Chinese Academy of Sciences, Beijing 100049, China;

    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China,University of Chinese Academy of Sciences, Beijing 100049, China;

    State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China,University of Chinese Academy of Sciences, Beijing 100049, China;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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