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首页> 外文期刊>Environmental Science & Technology >Occurrence and In Vitro Bioactivity of Estrogen, Androgen, and Glucocorticoid Compounds in a Nationwide Screen of United States Stream Waters
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Occurrence and In Vitro Bioactivity of Estrogen, Androgen, and Glucocorticoid Compounds in a Nationwide Screen of United States Stream Waters

机译：美国溪水全国筛查中的雌激素，雄激素和糖皮质激素化合物的发生和体外生物活性

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摘要

In vitro bioassays are sensitive, effect-based tools used to quantitatively screen for chemicals with nuclear receptor activity in environmental samples. We measured in vitro estrogen (ER), androgen (AR), and glucocorticoid receptor (GR) activity, along with a broad suite of chemical analytes, in streamwater from 35 well-characterized sites (3 reference and 32 impacted) across 24 states and Puerto Rico. ER agonism was the most frequently detected with nearly all sites (34/35) displaying activity (range, 0.054-116 ng E2Eq L~(-1))). There was a strong linear relationship (r~2 = 0.917) between in vitro ER activity and concentrations of steroidal estrogens after correcting for the in vitro potency of each compound. AR agonism was detected in 5/35 samples (range, 1.6-4.8 ng DHTEq L~(-1)) but concentrations of androgenic compounds were largely unable to account for the in vitro activity. Similarly, GR agonism was detected in 9/35 samples (range, 6.0-43 ng DexEq L~(-1))); however, none of the recognized GR-active compounds on the target-chemical analyte list were detected. The utility of in vitro assays in water quality monitoring was evident from both the quantitative agreement between ER activity and estrogen concentrations, as well as the detection of AR and GR activity for which there were limited or no corresponding target-chemical detections to explain the bioactivity. Incorporation of in vitro bioassays as complements to chemical analyses in standard water quality monitoring efforts would allow for more complete assessment of the chemical mixtures present in many surface waters.
机译：体外生物测定法是敏感的，基于效果的工具，用于定量筛选环境样品中具有核受体活性的化学物质。我们测量了来自24个州和地区的35个特征明确的地点（3个参考点和32个受影响的点）的废水中的体外雌激素（ER），雄激素（AR）和糖皮质激素受体（GR）活性，以及​​各种化学分析物。波多黎各。 ER激动是最常检测到的，几乎所有位点（34/35）都显示出活性（范围0.054-116 ng E2Eq L〜（-1））。校正每种化合物的体外效价后，体外ER活性与甾体雌激素浓度之间存在很强的线性关系（r〜2 = 0.917）。在5/35个样品（范围1.6-4.8 ng DHTEq L〜（-1））中检测到了AR激动作用，但雄激素化合物的浓度在很大程度上无法解释其体外活性。同样，在9/35个样本中检测到GR激动（范围6.0-43 ng DexEq L〜（-1）））；但是，在目标化学分析物列表中未检测到公认的GR活性化合物。从ER活性和雌激素浓度之间的定量协议以及对AR和GR活性的检测中，有限或没有相应的目标化学检测可以解释其生物活性，这证明了体外测定在水质监测中的实用性。在标准的水质监测工作中，将体外生物测定法作为化学分析的补充，可以对许多地表水中存在的化学混合物进行更完整的评估。

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  • 来源
    《Environmental Science & Technology》 |2017年第9期|4781-4791|共11页
  • 作者

    Justin M. Conley; Nicola Evans; Mary C. Cardón; Laura Rosenblum; Luke R Iwanowicz; Phillip C. Hartig; Kathleen M. Schenck; Paul M. Bradley; Vickie S. Wilson;

  • 作者单位

    U.S. Environmental Protection Agency/National HealthEnvironmental Effects Research Laboratory/Toxicity Assessment Division, Research Triangle Park, North Carolina 27711 United States;

    U.S. Environmental Protection Agency/National HealthEnvironmental Effects Research Laboratory/Toxicity Assessment Division, Research Triangle Park, North Carolina 27711 United States;

    U.S. Environmental Protection Agency/National HealthEnvironmental Effects Research Laboratory/Toxicity Assessment Division, Research Triangle Park, North Carolina 27711 United States;

    CB&I Federal Services, Cincinnati, Ohio 45212 United States;

    U.S. Geological Survey/Leetown Science Center, Kearneysville, West Virginia 25430 United States;

    U.S. Environmental Protection Agency/National HealthEnvironmental Effects Research Laboratory/Toxicity Assessment Division, Research Triangle Park, North Carolina 27711 United States;

    U.S. Environmental Protection Agency/National Risk Management Research Laboratory/Water Supply and Water Resources Division, Cincinnati, Ohio 45220 United States;

    U.S. Geological Survey/South Atlantic Water Science Center, Columbia, South Carolina 29210 United States;

    U.S. Environmental Protection Agency/National HealthEnvironmental Effects Research Laboratory/Toxicity Assessment Division, Research Triangle Park, North Carolina 27711 United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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