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首页> 外文期刊>Environmental Science & Technology >Rethinking Dithiothreitol-Based Paniculate Matter Oxidative Potential: Measuring Dithiothreitol Consumption versus Reactive Oxygen Species Generation
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Rethinking Dithiothreitol-Based Paniculate Matter Oxidative Potential: Measuring Dithiothreitol Consumption versus Reactive Oxygen Species Generation

机译：重新思考基于二硫苏糖醇的颗粒物质的氧化潜力：测量二硫苏糖醇的消耗量与活性氧的产生

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摘要

We measured the rate of generation of reactive oxygen species (ROS) [hydroxyl radicals (`OH) and hydrogen peroxide (H_2O_2)] catalyzed by ambient participate matter (PM) in the dithiothreitol (DTT) assay. To understand the mechanism of ROS generation, we tested several redox-active substances, such as 9,10-phenanthrenequinone (PQ), 5-hydroxy-1,4-naphthoquinone (5H-1,4NQ), 1,2-naphthoquinone (1,2-NQ), 1,4-naphthoquinone (1,4-NQ), copper(II), manganese(II), and iron (II and III). Both pure compounds and their mixtures show different patterns in DTT oxidation versus ROS generation. The quinones, known to oxidize DTT in the efficiency order of PQ> 5H-1,4NQ> 1,2-NQ> 1,4-NQ, show a different efficiency order (SH-1,4NQ> 1,2-NQ≈ PQ> 1,4-NQ) in the ROS generation. Cu(II), a dominant metal in DTT oxidation, contributes almost negligibly to the ROS generation. Fe is mostly inactive in DTT oxidation, but shows synergistic effect in ·OH formation in the presence of other quinones (mixture/sum > 1.5). Ten ambient PM samples collected from an urban site were analyzed, and although DTT oxidation was significantly correlated with H_2O_2 generation (Pearson's r = 0.91), no correlation was observed between DTT oxidation and ·OH formation. Our results show that measuring both DTT consumption and ROS generation in the DTT assay is important to incorporate the synergistic contribution from different aerosol components and to provide a more inclusive picture of the ROS activity of ambient PM.
机译：我们在二硫苏糖醇（DTT）分析中测量了环境参与物（PM）催化的活性氧（ROS）[羟基自由基（`OH）和过氧化氢（H_2O_2）]的生成速率。为了了解ROS的生成机理，我们测试了几种氧化还原活性物质，例如9,10-菲醌（PQ），5-羟基-1,4-萘醌（5H-1,4NQ），1,2-萘醌（ 1,2-NQ），1,4-萘醌（1,4-NQ），铜（II），锰（II）和铁（II和III）。纯化合物及其混合物在DTT氧化与ROS生成方面均显示出不同的模式。已知以PQ> 5H-1,4NQ> 1,2-NQ> 1,4-NQ的效率顺序氧化DTT的醌显示出不同的效率顺序（SH-1,4NQ>1,2-NQ≈ PQ> 1,4-NQ）。在DTT氧化中占主导地位的金属Cu（II）对ROS的贡献几乎可以忽略不计。 Fe在DTT氧化中大部分没有活性，但在其他醌存在下（•混合/总和> 1.5），在·OH形成中显示出协同作用。分析了从一个城市地点收集的十个环境PM样品，尽管DTT氧化与H_2O_2的生成显着相关（Pearson r = 0.91），但在DTT氧化与·OH的形成之间没有相关性。我们的结果表明，在DTT分析中测量DTT消耗量和ROS生成对于整合不同气溶胶成分的协同作用并提供环境PM的ROS活性更具包容性的图片非常重要。

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  • 来源
    《Environmental Science & Technology》 |2017年第11期|6507-6514|共8页
  • 作者

    Qianshan Xiong; Haoran Yu; Runran Wang; Jinlai Wei; Vishal Verma;

  • 作者单位

    Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, 205 North Mathews Avenue,Urbana, Illinois 61801, United States;

    Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, 205 North Mathews Avenue,Urbana, Illinois 61801, United States;

    Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, 205 North Mathews Avenue,Urbana, Illinois 61801, United States;

    Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, 205 North Mathews Avenue,Urbana, Illinois 61801, United States;

    Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, 205 North Mathews Avenue,Urbana, Illinois 61801, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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