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Identification of Chemicals that Cause Oxidative Stress in Oil Sands Process-Affected Water

机译:识别在油砂工艺影响的水中引起氧化应激的化学物质

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摘要

Oil sands process-affected water (OSPW) has been reported to cause oxidative stress in organisms, yet the causative agents remain unknown. In this study, a high-throughput in vitro Nrf2 reporter system was used, to determine chemicals in OSPW that cause oxidative stress. Five fractions, with increasing polarity, of the dissolved organic phase of OSPW were generated by use of solid phase extraction cartridges. The greatest response of Nrf2 was elicited by F2 (2.7 ± 0.1-fold), consistent with greater hydroperoxidation of lipids in embryos of Japanese medaka (Otyzias latipes) exposed to F2. Classic naphthenic acids were mainly eluted in Fl, and should not be causative chemicals. When F2 was fractionated into 60 subtractions by use of HPLC, significant activation of Nrf2 was observed in three grouped fractions: F2.8 (1.30 ± 0.01-fold), F2.16 (1.34 ± 0.05-fold), and F2.25 (1.28 ± 0.15-fold). 54 compounds were predicted to be potential chemicals causing Nrf2 response, predominated by S03+ and 03+ species. By use of high-resolution MS2 spectra, these SC*3+ and 03+ species were identified as hydroxylated aldehydes. This study demonstrated that polyoxygenated chemicals, rather than classic NAs, were the major chemicals responsible for oxidative stress in the aqueous phase of OSPW.
机译:据报道,受油砂工艺影响的水(OSPW)会引起生物体的氧化应激,但其致病因素仍然未知。在这项研究中,使用了高通量的体外Nrf2报告系统,以确定OSPW中引起氧化应激的化学物质。通过使用固相萃取柱,生成了极性增加的OSPW溶解有机相的五个馏分。 Nrf2的最大反应是由F2引起的(2.7±0.1倍),这与暴露于F2的日本(Otyzias latipes)胚胎中脂质的更大的氢过氧化作用相一致。经典的环烷酸主要在F1中洗脱,并且不应是致病性化学物质。当使用HPLC将F2分为60个减数时,在三组馏分中观察到Nrf2的显着活化:F2.8(1.30±0.01倍),F2.16(1.34±0.05倍)和F2.25( 1.28±0.15倍)。据预测,有54种化合物是引起Nrf2响应的潜在化学物质,主要由SO3 +和03+物种组成。通过使用高分辨率MS2光谱,这些SC * 3 +和03+物种被鉴定为羟基醛。这项研究表明,多加氧化学物质而不是经典的NAs是OSPW水相中引起氧化应激的主要化学物质。

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  • 来源
    《Environmental Science & Technology》 |2017年第15期|8773-8781|共9页
  • 作者单位

    Toxicology Centre, University of Saskatchewan, 44 Campus Drive, Saskatoon, Saskatchewan Canada;

    Toxicology Centre, University of Saskatchewan, 44 Campus Drive, Saskatoon, Saskatchewan Canada;

    Toxicology Centre, University of Saskatchewan, 44 Campus Drive, Saskatoon, Saskatchewan Canada,Schoo! of Environment and Sustainability, 117 Science Place, Saskatoon, Saskatchewan Canada;

    Schoo! of Environment and Sustainability, 117 Science Place, Saskatoon, Saskatchewan Canada,Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Saskatchewan Canada,Zoology Department, Center for Integrative Toxicology, Michigan State University, East Lansing, Michigan United States,School of Biological Sciences, University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China,State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing, People's Republic of China,Biology Department, Hong Kong Baptist University, Hong Kong, SAR, China;

    Department of Biological Sciences, University of Lethbridge, Lethbridge, Alberta T1K 3M4, Canada;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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  • 正文语种 eng
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