• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental Science & Technology >Monitoring Microbial Mineralization Using Reverse Stable Isotope Labeling Analysis by Mid-Infrared Laser Spectroscopy
【24h】

Monitoring Microbial Mineralization Using Reverse Stable Isotope Labeling Analysis by Mid-Infrared Laser Spectroscopy

机译：使用中红外激光光谱技术通过反向稳定同位素标记分析监测微生物矿化作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Assessing the biodegradation of organic compounds is a frequent question in environmental science. Here, we present a sensitive, inexpensive, and simple approach to monitor microbial mineralization using reverse stable isotope labeling analysis (RIL) of dissolved inorganic carbon (DIC). The medium for the biodegradation assay contains regular organic compounds and ~(13)C-labeled DIC with ~(13)C atom fractions (x(~(13)C)_(DIC)) higher than natural abundance (typically 2-50%). The produced CO_2 (x(~(13)C) ≈ 1.11%) gradually dilutes the initial x(~(13)C)_(DIC) allowing to quantify microbial mineralization using mass-balance calculations. For ~(13)C-enriched CO_2 samples, a newly developed isotope ratio mid-infrared spectrometer was introduced with a precision of x(~(13)C) < 0.006%. As an example for extremely difficult and slowly degradable compounds, CO_2 production was close to the theoretical stoichiometry for anaerobic naphthalene degradation by a sulfate-reducing enrichment culture. Furthermore, we could measure the aerobic degradation of dissolved organic carbon (DOC) adsorbed to granular activated carbon in a drinking water production plant, which cannot be labeled with ~(13)C. Thus, the RIL approach can be applied to sensitively monitor biodegradation of various organic compounds under anoxic or oxic conditions.
机译：评估有机化合物的生物降解是环境科学中的常见问题。在这里，我们提出了一种灵敏，廉价且简单的方法来使用溶解无机碳（DIC）的反向稳定同位素标记分析（RIL）监测微生物矿化作用。用于生物降解测定的介质包含常规有机化合物和〜（13）C标记的DIC，其〜（13）C原子分数（x（〜（13）C）_（DIC））高于自然丰度（通常为2-50） ％）。产生的CO_2（x（〜（13）C）≈1.11％）逐渐稀释了初始x（〜（13）C）_（DIC），从而可以使用质量平衡计算来量化微生物的矿化作用。对于〜（13）C富集的CO_2样品，引入了一种新开发的同位素比中红外光谱仪，其精度为x（〜（13）C）<0.006％。作为极其困难且缓慢降解的化合物的一个例子，CO_2的产量接近于通过减少硫酸盐的富集培养进行厌氧萘降解的理论化学计量。此外，我们可以测量饮用水生产厂中吸附到颗粒状活性炭上的溶解性有机碳（DOC）的好氧降解，这无法用〜（13）C进行标记。因此，RIL方法可用于在缺氧或有氧条件下灵敏地监测各种有机化合物的生物降解。

著录项

  • 来源
    《Environmental Science & Technology》 |2017年第20期|11876-11883|共8页
  • 作者

    Dong Xiyang; Jochmann Maik André; Elsner Martin; Meyer Armin H.; Bäcker Leonard E.; Rahmatullah Mona; Schunk Daniel; Lens Guido; Meckenstock Rainer U.;

  • 作者单位

    Biofilm Centre, University of Duisburg-Essen, Universitätsstrasse 5, Essen, Germany,Institute of Groundwater Ecology, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, Neuherberg, Germany;

    Instrumental Analytical Chemistry, University of Duisburg-Essen, Universitätsstr. 5, Essen, Germany;

    Institute of Groundwater Ecology, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, Neuherberg, Germany,Department of Analytical Chemistry and Water Chemistry, Technical University of Munich, Marchioninistrasse 17, Munich, Germany;

    Institute of Groundwater Ecology, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, Neuherberg, Germany;

    Biofilm Centre, University of Duisburg-Essen, Universitätsstrasse 5, Essen, Germany;

    Biofilm Centre, University of Duisburg-Essen, Universitätsstrasse 5, Essen, Germany;

    RWW Rheinisch-Westfälische Wasserwerksgesellschaft MbH, Am Schloß Broich 1-3, Mülheim an der Ruhr, Germany;

    RWW Rheinisch-Westfälische Wasserwerksgesellschaft MbH, Am Schloß Broich 1-3, Mülheim an der Ruhr, Germany;

    Biofilm Centre, University of Duisburg-Essen, Universitätsstrasse 5, Essen, Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号