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首页> 外文期刊>The Science of the Total Environment >Applying reverse stable isotope labeling analysis by mid-infrared laser spectroscopy to monitor BDOC in recycled wastewater
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Applying reverse stable isotope labeling analysis by mid-infrared laser spectroscopy to monitor BDOC in recycled wastewater

机译：中红外激光光谱应用反向稳定同位素标记分析，以监测再生废水中的BDOC

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Biological stability of treated wastewater is currently determined by methods such as biological oxygen demand, ATP-quantification, or flow-cytometric cell counting. However, the continuous increase in water reclamation for wastewater reuse requires new methods for quantifying degradation of biodegradable dissolved organic carbon (BDOC) ranging from very small to high concentrations of dissolved organic carbon (DOC). Furthermore, direct activity measures or absolute concentrations of BDOC are needed that produce comparable and reproducible results in all laboratories. Measuring carbon mineralization by CO2 evolution presents a suitable approach for directly measuring the microbial degradation activity. In this work, we investigated the extent of BDOC in water samples from effluent of a wastewater treatment plant and after purification by ultrafiltration over 204 days. BDOC monitoring was performed with the recently introduced reverse stable isotope labeling (RIL) analysis using mid-infrared spectroscopy for the monitoring of microbial CO2 production. Average BDOC degradation rates ranged from 0.11 to 0.32 mg L-1 d(-1) for wastewater treatment plant effluent and from 0.03 to 0.22 mg L-1 d(-1) after ultrafiltration. BDOC was degraded over 90 days indicating the long-term instability of the DOC. Degradation experiments over 88 days revealed first order kinetic rate constants for BDOC which corresponded to 12.7 . 10(-3) d(-1) for wastewater treatment plant effluent and 2.7 . 10(-3) d(-1) after ultrafiltration, respectively. A thorough sensitivity analysis of the RIL showed that the method is very accurate and sensitive with method detection limits down to 10 mu g . L-1 of measured CO2. (c) 2019 Elsevier B.V. All rights reserved.
机译：经处理废水的生物稳定性目前通过诸如生物需氧，ATP定量或流量 - 细胞术细胞计数的方法确定。然而，用于废水再利用的水填充的连续增加需要用于量化可生物降解的溶解有机碳（BDOC）的降解的新方法，范围为高浓度的溶解有机碳（DOC）。此外，需要直接活动措施或BDOC的绝对浓度，从而产生所有实验室的可比性和可重复的结果。通过CO2进化测量碳矿化具有直接测量微生物降解活性的合适方法。在这项工作中，我们研究了废水处理厂的流出物和204天内超滤后纯化后水样中BDOC的范围。使用中红外光谱法使用最近引入的反向稳定同位素标记（RIL）分析进行BDOC监测，用于监测微生物二氧化碳生产。平均BDOC降解速率范围为0.11至0.32mg L-1 d（-1），用于废水处理植物流出物和超滤后的0.03至0.22mg L-1 d（-1）。 BDOC降解过> 90天，表明DOC的长期不稳定性。 88天内的降解实验显示了与12.7相对应的BDOC的一级动力速率常数。 10（-3）D（-1）废水处理厂流出物和2.7。分别超滤后10（-3）D（-1）。 RIL的彻底敏感性分析表明，该方法非常精确，对方法检测限值降至10μg。测量CO2的L-1。 （c）2019 Elsevier B.v.保留所有权利。

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