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首页> 外文期刊>Environmental Science & Technology >Knocking Out OsPT4 Gene Decreases Arsenate Uptake by Rice Plants and Inorganic Arsenic Accumulation in Rice Grains
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Knocking Out OsPT4 Gene Decreases Arsenate Uptake by Rice Plants and Inorganic Arsenic Accumulation in Rice Grains

机译：敲除OsPT4基因可减少水稻植物吸收砷和水稻籽粒中无机砷的积累。

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摘要

Arsenic (As) accumulation in rice grains poses health risk to humans. Plants including rice take up arsenate (AsV) by phosphate transporters. In this study, rice phosphate transporter OsPT4 (OsPhtl;4) was investigated based on two independent T-DNA insertion mutants of OsPT4 (Ml and M2), which displayed stronger AsV resistance than wild types WTl and WT2. When cultivated in medium (+P or -P) with AsV, ospt4 mutants accumulated 16—32% lower As in plants, suggesting that OsPT4 mediates AsV uptake. Analysis of the xylem sap showed that AsV concentrations in ospt4 mutants was 20—40% lower than WT controls under -P condition, indicating OsPT4 may also mediate AsV translocation. Moreover, kinetics analysis showed that ospt4 mutants had lower AsV uptake rates than the WT controls, further proving that OsPT4 functions as an AsV transporter in rice. When grown in flooded soils with As, AsV concentrations in rice grains of ospt4 mutants decreased by 50—55%. More importantly, knocking out OsPT4 in Ml and M2 reduced inorganic As accumulation in rice grains by 20—44%, significant for controlling As exposure risk from rice. Taken together, our findings revealed a critical role of OsPT4 in AsV uptake and translocation in rice. Knocking out OsPT4 effectively decreased inorganic As accumulation in rice grains, shedding light on engineering low-As rice to enhance food safety.
机译：稻米中砷（As）的积累对人类健康构成威胁。包括水稻在内的植物通过磷酸盐转运蛋白吸收砷（AsV）。在该研究中，基于OsPT4的两个独立的T-DNA插入突变体（M1和M2），研究了水稻磷酸转运蛋白OsPT4（OsPhtl； 4），其显示出比野生型WT1和WT2更强的AsV抗性。当在带有AsV的培养基（+ P或-P）中培养时，ospt4突变体的植物中As的积累量降低了16-32％，这表明OsPT4介导了AsV的吸收。对木质部汁液的分析表明，在-P条件下，ospt4突变体中的AsV浓度比野生型对照低20-40％，这表明OsPT4也可能介导AsV易位。此外，动力学分析表明，ospt4突变体比野生型对照具有较低的AsV吸收率，进一步证明OsPT4可以作为水稻中的AsV转运蛋白。当在充满As的淹水土壤中生长时，ospt4突变体的水稻籽粒中的AsV浓度降低了50-55％。更重要的是，敲除M1和M2中的OsPT4可使水稻籽粒中无机砷的积累减少20-44％，对于控制水稻中砷的暴露风险具有重要意义。综上所述，我们的发现揭示了OsPT4在水稻中AsV吸收和转运中的关键作用。剔除OsPT4可有效减少水稻籽粒中无机砷的积累，从而减轻了对低砷稻谷工程化工作的负担，从而提高了食品安全性。

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  • 来源
    《Environmental Science & Technology》 |2017年第21期|12131-12138|共8页
  • 作者

    Yue Cao; Dan Sun; Hao Ai; Hanyi Mei; Xue Liu; Shubin Sun; Guohua Xu; Yungen Liu; Yanshan Chen; Lena Q Ma;

  • 作者单位

    State Key Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing Jiangsu 210023, China;

    State Key Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing Jiangsu 210023, China;

    State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China;

    State Key Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing Jiangsu 210023, China;

    State Key Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing Jiangsu 210023, China;

    State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China;

    State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China;

    Research Institute of Rural Sewage Treatment, South West Forestry University, Kunming, Yunnan 650224, China;

    State Key Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing Jiangsu 210023, China;

    State Key Lab of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing Jiangsu 210023, China,Soil and Water Science Department, University of Florida, Gainesville, Florida 32611, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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