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首页> 外文期刊>Environmental Science & Technology >Relative Contribution of nirK- and nirS- Bacterial Denitrifiers as Well as Fungal Denitrifiers to Nitrous Oxide Production from Dairy Manure Compost
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Relative Contribution of nirK- and nirS- Bacterial Denitrifiers as Well as Fungal Denitrifiers to Nitrous Oxide Production from Dairy Manure Compost

机译：nirK-和nirS-细菌反硝化剂以及真菌反硝化剂对奶牛粪便堆肥生产一氧化二氮的相对贡献

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摘要

The relative contribution of fungi, bacteria, and nirS and nirK denirifiers to nitrous oxide (N2O) emission with unknown isotopic signature from dairy manure compost was examined by selective inhibition techniques. Chloramphenicol (CHP), cycloheximide (CYH), and diethyl dithiocarbamate (DDTC) were used to suppress the activity of bacteria, fungi, and nirK-possessing denitrifiers, respectively. Produced N2O were surveyed to isotopocule analysis, and its N-15 site preference (SP) and delta O-18 values were compared. Bacteria, fungi, nirS, and nirK gene abundances were compared by qPCR. The results showed that N2O production was strongly inhibited by CHP addition in surface pile samples (82.2%) as well as in nitrite-amended core samples (98.4%), while CYH addition did not inhibit the N2O production. N2O with unknown isotopic signature (SP = 15.3-16.2), accompanied by delta O-18 (19.0-26.8) values which were close to bacterial denitrification, was also suppressed by CHP and DDTC addition (95.3%) indicating that nirK denitrifiers were responsible for this N2O production despite being less abundant than nirS denitrifiers. Altogether, our results suggest that bacteria are important for N2O production with different SP values both from compost surface and pile core. However, further work is required to decipher whether N2O with unknown isotopic signature is mostly due to nirK denitrifiers that are taxonomically different from the SP-characterized strains and therefore have different SP values rather than also being interwoven with the contribution of the NO-detoxifying pathway and/or of co-denitrification.
机译：通过选择性抑制技术检查了真菌，细菌以及nirS和nirK改性剂对奶牛粪便堆肥中具有未知同位素特征的一氧化二氮（N2O）排放的相对贡献。氯霉素（CHP），环己酰亚胺（CYH）和二乙基二硫代氨基甲酸酯（DDTC）分别用于抑制细菌，真菌和具有nirK的反硝化剂的活性。对产生的N2O进行同位素同位素分析，并比较其N-15位点偏好（SP）和δO-18值。通过qPCR比较细菌，真菌，nirS和nirK基因的丰度。结果表明，表面堆样品（82.2％）和亚硝酸盐修饰岩心样品（98.4％）中的CHP加入会强烈抑制N2O的产生，而CYH的加入不会抑制N2O的产生。带有未知同位素特征的N2O（SP = 15.3-16.2），伴随着接近细菌反硝化作用的δO-18值（19.0-26.8），也被CHP和DDTC的添加（95.3％）抑制，表明nirK反硝化剂起作用尽管不如nirS反硝化器丰富，但仍可用于生产N2O。总而言之，我们的结果表明，细菌对于堆肥表面和堆芯处具有不同SP值的N2O生产很重要。但是，需要进一步的工作来判别具有未知同位素特征的N2O是否主要是由于nirK脱氮剂在分类学上不同于SP表征的菌株，因此具有不同的SP值，而不是与NO脱毒途径的影响交织在一起的和/或共同脱硝化。

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  • 来源
    《Environmental Science & Technology》 |2017年第24期|14083-14091|共9页
  • 作者

    Maeda Koki; Toyoda Sakae; Philippot Laurent; Hattori Shohei; Nakajima Keiichi; Ito Yumi; Yoshida Naohiro;

  • 作者单位

    NARO, Hokkaido Agr Res Ctr, Dairy Res Div, 1 Hitsujigaoka, Sapporo, Hokkaido 0628555, Japan;

    Tokyo Inst Technol, Sch Mat & Chem Technol, Dept Chem Sci & Engn, Midori Ku, 4259 Nagatsuta, Yokohama, Kanagawa 2268502, Japan;

    INRA, UMR 1229, Soil & Environm Microbiol, 17 Rue Sully,BP 86510, F-21065 Dijon, France;

    Tokyo Inst Technol, Sch Mat & Chem Technol, Dept Chem Sci & Engn, Midori Ku, 4259 Nagatsuta, Yokohama, Kanagawa 2268502, Japan;

    NARO, Hokkaido Agr Res Ctr, Dairy Res Div, 1 Hitsujigaoka, Sapporo, Hokkaido 0628555, Japan;

    NARO, Hokkaido Agr Res Ctr, Dairy Res Div, 1 Hitsujigaoka, Sapporo, Hokkaido 0628555, Japan;

    Tokyo Inst Technol, Sch Mat & Chem Technol, Dept Chem Sci & Engn, Midori Ku, 4259 Nagatsuta, Yokohama, Kanagawa 2268502, Japan;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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