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首页> 外文期刊>Environmental Science & Technology >Characterizing Bacteriophage PR772 as a Potential Surrogate for Adenovirus in Water Disinfection: A Comparative Analysis of Inactivation Kinetics and Replication Cycle Inhibition by Free Chlorine
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Characterizing Bacteriophage PR772 as a Potential Surrogate for Adenovirus in Water Disinfection: A Comparative Analysis of Inactivation Kinetics and Replication Cycle Inhibition by Free Chlorine

机译：表征噬菌体PR772作为水消毒中腺病毒的潜在替代物：游离氯的灭活动力学和复制周期抑制的比较分析

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摘要

Elucidating mechanisms by which pathogenic waterbome viruses become inactivated by drinking water disinfectants would facilitate the development of sensors to detect infectious viruses and novel disinfection strategies to provide safe water. Using bacteriophages as surrogates for human pathogenic viruses could assist in elucidating these mechanisms; however, an appropriate viral surrogate for human adenovirus (HAdV), a medium-sized virus with a double-stranded DNA genome, needs to be identified. Here, we characterized the inactivation kinetics of bacteriophage PR772, a member of the Tectiviridae family with many similarities in structure and replication to HAdV. The inactivation of PR772 and HAdV by free chlorine had similar kinetics that could be represented with a model previously developed for HAdV type 2 (HAdV-2). We developed and tested a quantitative assay to analyze several steps in the PR772 replication cycle to determine if both viruses being inactivated at similar rates resulted from similar replication cycle events being inhibited. Like HAdV-2, we observed that PR772 inactivated by free chlorine still attached to host cells, and viral DNA synthesis and early and late gene transcription were inhibited. Consequently, free chlorine exposure inhibited a replication cycle event that was post-binding but took place prior to early gene synthesis for both PR772 and HAdV-2.
机译：阐明病原性水疱病毒被饮用水消毒剂灭活的机制，将有助于开发检测传染性病毒的传感器和提供安全水的新型消毒策略。使用噬菌体作为人类病原性病毒的替代物可以帮助阐明这些机制。但是，需要确定一种适合人类腺病毒（HAdV）的病毒替代物，HAdV是一种具有双链DNA基因组的中型病毒。在这里，我们表征了噬菌体PR772的灭活动力学，该噬菌体是Tectiviridae家族的成员，其结构和复制与HAdV相似。游离氯对PR772和HAdV的灭活具有相似的动力学，可以用先前针对2型HAdV开发的模型（HAdV-2）表示。我们开发并测试了一种定量分析方法，以分析PR772复制周期中的几个步骤，以确定两种病毒是否都以相似的速率被灭活，这是由于相似的复制周期事件被抑制所致。像HAdV-2一样，我们观察到PR772被仍然附着在宿主细胞上的游离氯所灭活，并且病毒DNA的合成以及早期和晚期基因转录都受到抑制。因此，游离氯暴露抑制了复制循环事件，该事件是结合后的，但发生在PR772和HAdV-2的早期基因合成之前。

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  • 来源
    《Environmental Science & Technology》 |2016年第5期|2522-2529|共8页
  • 作者

    Aimee M. Gall; Joanna L. Shisler; Benito J. Marinas;

  • 作者单位

    Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States,Safe Global Water Institute, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States;

    Department of Microbiology and College of Medicine, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States,Safe Global Water Institute, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States;

    Department of Civil and Environmental Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States,Safe Global Water Institute, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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