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首页> 外文期刊>Environmental Science & Technology >Detection of the Antimicrobial Triclosan in Environmental Samples by Immunoassay
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Detection of the Antimicrobial Triclosan in Environmental Samples by Immunoassay

机译：免疫测定法检测环境样品中的抗菌三氯生

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摘要

A sensitive, competitive enzyme-linked immu- nosorbent assay (ELISA) for the detection of the antimicrobial triclosan (TCS; 2,4,4'-trichloro-2'-hydroxydiphenyl ether) was developed. Novel immunizing haptens were synthesized by derivatizing at the 4-Cl position of the TCS molecule. Compounds derived from substitutions at 4'-Cl and that replaced the 2'-OH with a Cl atom were designed as unique coating antigen haptens. Polydonal rabbit antisera were screened against the coating antigen library to identify combinations of immunoreagents resulting in the most sensitive assays. The most sensitive assay identified was one utilizing antiserum no. 1155 and a heterologous competitive hapten, where the 2'-OH group was substituted with a Cl atom. An IC_(50) value and the detection range for TCS in assay buffer were 1.19 and 0.21-6.71 μg/L, respectively. The assay was selective for TCS, providing low cross-reactivity (＜5%) to the major metabolites of TCS and to brominated diphenyl ether-47. A second assay utilizing a competitive hapten containing Br instead of Cl substitutions was broadly selective for both brominated and chlorinated diphenylethers. Using the most sensitive assay combination, we measured TCS concentrations in water samples following dilution. Biosolid samples were analyzed following the dilution of a simple solvent extract. The immunoassay results were similar to those determined by LC-MS/MS. This immunoassay can be used as a rapid and convenient tool to screen for human and environmental exposure.
机译：开发了一种灵敏的，竞争性的酶联免疫吸附测定法（ELISA），用于检测抗菌三氯生（TCS; 2,4,4'-三氯-2'-羟基二苯醚）。通过在TCS分子的4-Cl位置衍生化来合成新型免疫半抗原。将源自4'-Cl取代并用Cl原子取代2'-OH的化合物设计为独特的包被抗原半抗原。针对包被抗原文库筛选了多齿兔抗血清，以鉴定免疫试剂的组合，从而得到最灵敏的检测方法。鉴定出的最灵敏的检测方法是利用抗血清No.1。 1155和异源竞争性半抗原，其中2'-OH基团被Cl原子取代。测定缓冲液中TCS的IC_（50）值和检测范围分别为1.19和0.21-6.71μg/ L。该方法对TCS具有选择性，对TCS的主要代谢产物和溴化二苯醚47的交叉反应性低（＜5％）。使用含有Br而不是Cl取代的竞争性半抗原的第二种测定法对溴化和氯化二苯醚均具有广泛的选择性。使用最敏感的测定组合，我们测量了稀释后水样品中的TCS浓度。稀释简单的溶剂提取物后，分析生物固体样品。免疫测定结果与通过LC-MS / MS测定的结果相似。该免疫测定可用作筛选人类和环境暴露的快速便捷工具。

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  • 来源
    《Environmental Science & Technology》 |2016年第7期|3754-3761|共8页
  • 作者

    Ki Chang Ahn; Anupama Ranganathan; Candace S. Bever; Sung Hee Hwang; Erika B. Holland; Kevin Morisseau; Isaac N. Pessah; Bruce D. Hammock; Shirley J. Gee;

  • 作者单位

    Department of Entomology and Nematology and UCD Comprehensive Cancer Center University of California Davis, Davis, California 95616, United States;

    Department of Entomology and Nematology and UCD Comprehensive Cancer Center University of California Davis, Davis, California 95616, United States;

    Department of Entomology and Nematology and UCD Comprehensive Cancer Center University of California Davis, Davis, California 95616, United States;

    Department of Entomology and Nematology and UCD Comprehensive Cancer Center University of California Davis, Davis, California 95616, United States;

    Department of Molecular Biosciences, University of California Davis, Davis, California 95616, United States;

    Department of Entomology and Nematology and UCD Comprehensive Cancer Center University of California Davis, Davis, California 95616, United States;

    Department of Molecular Biosciences, University of California Davis, Davis, California 95616, United States;

    Department of Entomology and Nematology and UCD Comprehensive Cancer Center University of California Davis, Davis, California 95616, United States;

    Department of Entomology and Nematology and UCD Comprehensive Cancer Center University of California Davis, Davis, California 95616, United States;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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